TY - JOUR
T1 - Cupriavidus alkaliphilus sp. nov., a new species associated with agricultural plants that grow in alkaline soils
AU - Estrada-de los Santos, Paulina
AU - Martínez-Aguilar, Lourdes
AU - López-Lara, Isabel M.
AU - Caballero-Mellado, Jesús
PY - 2012/7
Y1 - 2012/7
N2 - A group of 20 bacterial strains was isolated from the rhizosphere of different agricultural plants growing in alkaline soils in the northeast of Mexico. The phylogenetic analysis of the 16S rRNA gene sequence from four strains showed that this novel group belonged to the Cupriavidus genus, with C. taiwanensis (~98.9%) and C. necator (~98.8%) as the closest species. However, DNA-DNA reassociation values were less than 20%. The novel group did not fix nitrogen and lacked nifH and nodA genes, unlike C. taiwanensis. Whole-cell protein patterns were highly similar among the 20 strains but different from the closest Cupriavidus species. BOX-PCR patterns were distinct among the 20 strains but also differed from other Cupriavidus type species. The major cellular fatty acids from strains ASC-732T and SLV-2362 were C16:0, C18:1 ω7c/12t/9t and C16:1 ω7c and/or C15:0 iso 2OH. The major polar lipids consisted of phosphatidylglycerol, cardiolipin, phosphatidylethanolamine, 2-hydroxylated-phosphatidylethanolamine and an unknown aminolipid. The DNA G+C content of strain ASC-732T was 66.8mol%. All 20 strains grew in the presence of 5-10mgmL-1 arsenic, 1mgmL-1 zinc, and 0.1mgmL-1 copper. Consequently, the group of strains was considered to represent a novel species for which the name Cupriavidus alkaliphilus sp. nov. is proposed. The type strain is ASC-732T (=LMG 26294T=CIP 110330T).
AB - A group of 20 bacterial strains was isolated from the rhizosphere of different agricultural plants growing in alkaline soils in the northeast of Mexico. The phylogenetic analysis of the 16S rRNA gene sequence from four strains showed that this novel group belonged to the Cupriavidus genus, with C. taiwanensis (~98.9%) and C. necator (~98.8%) as the closest species. However, DNA-DNA reassociation values were less than 20%. The novel group did not fix nitrogen and lacked nifH and nodA genes, unlike C. taiwanensis. Whole-cell protein patterns were highly similar among the 20 strains but different from the closest Cupriavidus species. BOX-PCR patterns were distinct among the 20 strains but also differed from other Cupriavidus type species. The major cellular fatty acids from strains ASC-732T and SLV-2362 were C16:0, C18:1 ω7c/12t/9t and C16:1 ω7c and/or C15:0 iso 2OH. The major polar lipids consisted of phosphatidylglycerol, cardiolipin, phosphatidylethanolamine, 2-hydroxylated-phosphatidylethanolamine and an unknown aminolipid. The DNA G+C content of strain ASC-732T was 66.8mol%. All 20 strains grew in the presence of 5-10mgmL-1 arsenic, 1mgmL-1 zinc, and 0.1mgmL-1 copper. Consequently, the group of strains was considered to represent a novel species for which the name Cupriavidus alkaliphilus sp. nov. is proposed. The type strain is ASC-732T (=LMG 26294T=CIP 110330T).
KW - Alkaline
KW - BCCM/LMG
KW - CIP
KW - Cupriavidus
KW - Metal resistance
KW - Rhizosphere
KW - Soil pH
UR - http://www.scopus.com/inward/record.url?scp=84864309428&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2012.05.005
DO - 10.1016/j.syapm.2012.05.005
M3 - Artículo
SN - 0723-2020
VL - 35
SP - 310
EP - 314
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 5
ER -