Cuantificación de EPO humana recombinante por Fase Reversa-Cromatografía Líquida de Ultra Resolución

R. Emilio Medina; E. Carolina Ortiz; R. Eduardo González; O. Néstor; R. Pérez

Cuantificación de EPO humana recombinante por Fase Reversa-Cromatografía Líquida de Ultra Resolución

Translated title of the contribution: Quantification of recombinant human EPO by Reverse Phase Liquid Chromatography Ultra-Resolution

R. Emilio Medina, E. Carolina Ortiz, R. Eduardo González, O. Néstor, R. Pérez

Research output: Contribution to journal › Article › peer-review

Abstract

Recombinant human Erythropoietin (rhEPO) is produced through DNA recombinant technology in Chinese Hamster Ovary cells. Its quantification during the manufacturing process is usually performed with immunoassay techniques, which is expensive and time consuming. The present study proposes a methodology to quantify rhEPO by reverse phase-ultra performance liquid chromatography (RP-UPLC) in broad media culture and clarified media. The article presents the validation of the method that demonstrates its precision, accuracy and specificity with a CV below of 5%.

Translated title of the contribution	Quantification of recombinant human EPO by Reverse Phase Liquid Chromatography Ultra-Resolution
Original language	Spanish
Pages (from-to)	20-26
Number of pages	7
Journal	Revista Mexicana de Ciencias Farmaceuticas
Volume	42
Issue number	2
State	Published - Apr 2011
Externally published	Yes

Cite this

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title = "Cuantificaci{\'o}n de EPO humana recombinante por Fase Reversa-Cromatograf{\'i}a L{\'i}quida de Ultra Resoluci{\'o}n",

abstract = "Recombinant human Erythropoietin (rhEPO) is produced through DNA recombinant technology in Chinese Hamster Ovary cells. Its quantification during the manufacturing process is usually performed with immunoassay techniques, which is expensive and time consuming. The present study proposes a methodology to quantify rhEPO by reverse phase-ultra performance liquid chromatography (RP-UPLC) in broad media culture and clarified media. The article presents the validation of the method that demonstrates its precision, accuracy and specificity with a CV below of 5%.",

keywords = "Erythropoietin, Reverse phase, UPLC, Validation",

author = "{Emilio Medina}, R. and {Carolina Ortiz}, E. and {Eduardo Gonz{\'a}lez}, R. and O. N{\'e}stor and R. P{\'e}rez",

year = "2011",

month = apr,

language = "Espa{\~n}ol",

volume = "42",

pages = "20--26",

journal = "Revista Mexicana de Ciencias Farmaceuticas",

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TY - JOUR

T1 - Cuantificación de EPO humana recombinante por Fase Reversa-Cromatografía Líquida de Ultra Resolución

AU - Emilio Medina, R.

AU - Carolina Ortiz, E.

AU - Eduardo González, R.

AU - Néstor, O.

AU - Pérez, R.

PY - 2011/4

Y1 - 2011/4

N2 - Recombinant human Erythropoietin (rhEPO) is produced through DNA recombinant technology in Chinese Hamster Ovary cells. Its quantification during the manufacturing process is usually performed with immunoassay techniques, which is expensive and time consuming. The present study proposes a methodology to quantify rhEPO by reverse phase-ultra performance liquid chromatography (RP-UPLC) in broad media culture and clarified media. The article presents the validation of the method that demonstrates its precision, accuracy and specificity with a CV below of 5%.

AB - Recombinant human Erythropoietin (rhEPO) is produced through DNA recombinant technology in Chinese Hamster Ovary cells. Its quantification during the manufacturing process is usually performed with immunoassay techniques, which is expensive and time consuming. The present study proposes a methodology to quantify rhEPO by reverse phase-ultra performance liquid chromatography (RP-UPLC) in broad media culture and clarified media. The article presents the validation of the method that demonstrates its precision, accuracy and specificity with a CV below of 5%.

KW - Erythropoietin

KW - Reverse phase

KW - UPLC

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=84867671773&partnerID=8YFLogxK

M3 - Artículo

SN - 1027-3956

VL - 42

SP - 20

EP - 26

JO - Revista Mexicana de Ciencias Farmaceuticas

JF - Revista Mexicana de Ciencias Farmaceuticas

IS - 2

ER -

Cuantificación de EPO humana recombinante por Fase Reversa-Cromatografía Líquida de Ultra Resolución

Abstract

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