TY - JOUR
T1 - Construction of an electrochemical genosensor based on screen-printed gold electrodes (SPGE) for detection of a mutation in the adenomatous polyposis coli gene
AU - Garcia-Melo, Luis Fernando
AU - Álvarez-González, Isela
AU - Madrigal-Bujaidar, Eduardo
AU - Madrigal-Santillán, Eduardo O.
AU - Morales-González, José Antonio
AU - Pineda Cruces, Rosa Natali
AU - Campoy Ramírez, Jorge Alfredo
AU - Matsumura, Pablo Damian
AU - Aguilar-Santamaría, María de los Angeles
AU - Batina, Nikola
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Studies have well documented that mutations in the adenomatous polyposis coli gene (APC) are involved in the development of different types of cancer. Therefore, the objective of the present report was to design and construct a sensitive nanostructured genosensor able to detect specific sequences of the APC gene in order to be applied in early carcinogenesis diagnosis. We used screen-printed gold electrodes (SPGE) modified with DNA probes and mercaptoundecanoic acid chemically immobilized on a gold sensor surface. APC complementary DNA sequences were hybridized on the previously immobilized DNA probes. Besides, we tested effects of different intercalating agents, and various temperatures on the hybridization reaction to increase the sensitivity and selectivity of our device. The evaluation of these processes was carried out by means of the cyclic voltammetry technique (CV) to monitor the redox reaction of [Fe(CN) 6 ] 3−/4− on the SPGE-s. The constructed genosensor exhibited an excellent response to identify the APC gene in a concentration range from 100 pM to 100 μM, with 43.92 pM as the limit of detection in the presence of doxorubicin as DNA intercalating agent. The selectivity of the device was tested by using a DNA sequence different from the APC gene, which shows negative response. Considering the importance of APC mutations in the development of tumors in the digestive, urogenital, and mammary tissues, here developed electrochemical genosensor, due to its easy management, short time of response, and high performance, may be useful for early cancer detection.
AB - Studies have well documented that mutations in the adenomatous polyposis coli gene (APC) are involved in the development of different types of cancer. Therefore, the objective of the present report was to design and construct a sensitive nanostructured genosensor able to detect specific sequences of the APC gene in order to be applied in early carcinogenesis diagnosis. We used screen-printed gold electrodes (SPGE) modified with DNA probes and mercaptoundecanoic acid chemically immobilized on a gold sensor surface. APC complementary DNA sequences were hybridized on the previously immobilized DNA probes. Besides, we tested effects of different intercalating agents, and various temperatures on the hybridization reaction to increase the sensitivity and selectivity of our device. The evaluation of these processes was carried out by means of the cyclic voltammetry technique (CV) to monitor the redox reaction of [Fe(CN) 6 ] 3−/4− on the SPGE-s. The constructed genosensor exhibited an excellent response to identify the APC gene in a concentration range from 100 pM to 100 μM, with 43.92 pM as the limit of detection in the presence of doxorubicin as DNA intercalating agent. The selectivity of the device was tested by using a DNA sequence different from the APC gene, which shows negative response. Considering the importance of APC mutations in the development of tumors in the digestive, urogenital, and mammary tissues, here developed electrochemical genosensor, due to its easy management, short time of response, and high performance, may be useful for early cancer detection.
KW - APC genosensor
KW - Doxorubicin
KW - Electrochemical genosensor
KW - Gene detection
UR - http://www.scopus.com/inward/record.url?scp=85063720372&partnerID=8YFLogxK
U2 - 10.1016/j.jelechem.2019.03.048
DO - 10.1016/j.jelechem.2019.03.048
M3 - Artículo
AN - SCOPUS:85063720372
SN - 1572-6657
VL - 840
SP - 93
EP - 100
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
ER -