TY - JOUR
T1 - Constitutive and inducible pectinolytic enzymes from aspergillus flavipes fp-500 and their modulation by ph and carbon source
AU - Aurora, Martínez Trujillo
AU - Aranda, Juan S.
AU - Carlos, Gómez Sánchez
AU - Blanca, Trejo Aguilar
AU - Guillermo, Aguilar Osorio
PY - 2009
Y1 - 2009
N2 - Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase.
AB - Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be produced was exopectinase followed by Pectin Lyase and Endo-pectinase.
KW - Aspergillus flavipes
KW - Constitutive enzymes
KW - Inducible enzymes
KW - Pectin
KW - Pectinolytic enzymes
UR - http://www.scopus.com/inward/record.url?scp=67149144497&partnerID=8YFLogxK
U2 - 10.1590/s1517-83822009000100006
DO - 10.1590/s1517-83822009000100006
M3 - Artículo
SN - 1517-8382
VL - 40
SP - 40
EP - 47
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 1
ER -