TY - JOUR
T1 - Conditions that induce biofilm production by Ornithobacterium rhinotracheale
AU - De la Rosa-Ramos, Miguel A.
AU - Rodríguez-Cruz, Maricruz
AU - López-Villegas, Edgar O.
AU - Castro-Escarpulli, Graciela
AU - Guerra-Infante, Fernando M.
N1 - Publisher Copyright:
© 2015 Houghton Trust Ltd.
PY - 2015/9/3
Y1 - 2015/9/3
N2 - Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.
AB - Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.
UR - http://www.scopus.com/inward/record.url?scp=84943253138&partnerID=8YFLogxK
U2 - 10.1080/03079457.2015.1059923
DO - 10.1080/03079457.2015.1059923
M3 - Artículo
C2 - 26063103
SN - 0307-9457
VL - 44
SP - 366
EP - 369
JO - Avian Pathology
JF - Avian Pathology
IS - 5
ER -