Conditions that induce biofilm production by Ornithobacterium rhinotracheale

Miguel A. De la Rosa-Ramos, Maricruz Rodríguez-Cruz, Edgar O. López-Villegas, Graciela Castro-Escarpulli, Fernando M. Guerra-Infante

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

© 2015 Houghton Trust Ltd. Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.
Original languageAmerican English
Pages (from-to)366-369
Number of pages4
JournalAvian Pathology
DOIs
StatePublished - 1 Jan 2015

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Ornithobacterium
Ornithobacterium rhinotracheale
Biofilms
biofilm
Carbon Monoxide
serotypes
Bird Diseases
poultry industry
Polystyrenes
polystyrenes
Poultry
respiratory tract diseases
Bacillus
Industry
microorganisms

Cite this

De la Rosa-Ramos, M. A., Rodríguez-Cruz, M., López-Villegas, E. O., Castro-Escarpulli, G., & Guerra-Infante, F. M. (2015). Conditions that induce biofilm production by Ornithobacterium rhinotracheale. Avian Pathology, 366-369. https://doi.org/10.1080/03079457.2015.1059923
De la Rosa-Ramos, Miguel A. ; Rodríguez-Cruz, Maricruz ; López-Villegas, Edgar O. ; Castro-Escarpulli, Graciela ; Guerra-Infante, Fernando M. / Conditions that induce biofilm production by Ornithobacterium rhinotracheale. In: Avian Pathology. 2015 ; pp. 366-369.
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De la Rosa-Ramos, MA, Rodríguez-Cruz, M, López-Villegas, EO, Castro-Escarpulli, G & Guerra-Infante, FM 2015, 'Conditions that induce biofilm production by Ornithobacterium rhinotracheale', Avian Pathology, pp. 366-369. https://doi.org/10.1080/03079457.2015.1059923

Conditions that induce biofilm production by Ornithobacterium rhinotracheale. / De la Rosa-Ramos, Miguel A.; Rodríguez-Cruz, Maricruz; López-Villegas, Edgar O.; Castro-Escarpulli, Graciela; Guerra-Infante, Fernando M.

In: Avian Pathology, 01.01.2015, p. 366-369.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Conditions that induce biofilm production by Ornithobacterium rhinotracheale

AU - De la Rosa-Ramos, Miguel A.

AU - Rodríguez-Cruz, Maricruz

AU - López-Villegas, Edgar O.

AU - Castro-Escarpulli, Graciela

AU - Guerra-Infante, Fernando M.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © 2015 Houghton Trust Ltd. Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.

AB - © 2015 Houghton Trust Ltd. Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacillus that causes respiratory disease in birds, and directly affects the poultry industry. The mechanisms behind these infections are not completely known. Currently, its capacity to form biofilms on inert surfaces has been reported; however, the conditions for biofilm development have not been described yet. The present work was aimed at identifying the conditions that enhance in vitro biofilm formation and development by ORT. For this, serovars A-E were analysed to assess their ability to induce biofilm development on 96-well flat-bottom polystyrene microtitre plates under diverse conditions: temperature, incubation time, and CO 2 concentration. The results obtained showed not only that all serovars have the ability to produce in vitro biofilms, but also that the optimal conditions for biofilm density were 40°C after 72 h at an elevated CO 2 concentration. In conclusion, ORT biofilm formation depends on the environmental conditions and may contribute to the persistence of this microorganism.

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De la Rosa-Ramos MA, Rodríguez-Cruz M, López-Villegas EO, Castro-Escarpulli G, Guerra-Infante FM. Conditions that induce biofilm production by Ornithobacterium rhinotracheale. Avian Pathology. 2015 Jan 1;366-369. https://doi.org/10.1080/03079457.2015.1059923