TY - JOUR
T1 - Complete type III secretion system of a mesophilic Aeromonas hydrophila strain
AU - Vilches, Silvia
AU - Urgell, Cecilia
AU - Merino, Susana
AU - Chacón, Matilde R.
AU - Soler, Lara
AU - Castro-Escarpulli, Graciela
AU - Figueras, Maria Jose
AU - Tomás, Juan M.
PY - 2004/11
Y1 - 2004/11
N2 - We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa. Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila, A. veronii, and A. caviae. By using one of the TTSS genes (ascV), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.
AB - We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa. Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila, A. veronii, and A. caviae. By using one of the TTSS genes (ascV), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.
UR - http://www.scopus.com/inward/record.url?scp=8744303082&partnerID=8YFLogxK
U2 - 10.1128/AEM.70.11.6914-6919.2004
DO - 10.1128/AEM.70.11.6914-6919.2004
M3 - Artículo
SN - 0099-2240
VL - 70
SP - 6914
EP - 6919
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 11
ER -