TY - JOUR
T1 - Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species
AU - Baires-Varguez, Laura
AU - Cruz-García, Alejandro
AU - Villa-Tanaka, Lourdes
AU - Sánchez-García, Sergio
AU - Gaitán-Cepeda, Luis Alberto
AU - Sánchez-Vargas, Luis Octavio
AU - Quindós, Guillermo
AU - Hernández-Rodríguez, César
N1 - Funding Information:
The authors’ work was financed in part by the project PI030662/2003 and PI061895/2006 of the Fondo de Investigación Sanitaria del Ministerio de Sanidad de España and project UPV-GIU05/05 1/UPV 00093.327-E-14645/2002 of the Universidad del País Vasco-Euskal Herriko Unibertsitatea.
PY - 2007/6
Y1 - 2007/6
N2 - The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.
AB - The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.
KW - ATB ID32C
KW - Candida
KW - Clinical isolates
KW - Identification
KW - RAPD
UR - http://www.scopus.com/inward/record.url?scp=34347392892&partnerID=8YFLogxK
U2 - 10.1016/S1130-1406(07)70031-1
DO - 10.1016/S1130-1406(07)70031-1
M3 - Artículo
SN - 1130-1406
VL - 24
SP - 148
EP - 151
JO - Revista Iberoamericana de Micologia
JF - Revista Iberoamericana de Micologia
IS - 2
ER -