TY - JOUR
T1 - Comparative treatment with hyperbaric oxygen therapy in a model of systemic loxoscelism in rats
AU - Toledo-Blas, Mireille
AU - Franco-Vadillo, Antonio
AU - Somilleda-Ventura, Selma A.
AU - Dominguez-Ruiz, Brenda
AU - Guevara-Balcazar, Gustavo
AU - Kormanovski-Kovzova, Alexandre
AU - Lopez-Sanchez, Pedro
AU - Jarillo-Luna, Rosa A.
AU - Lara-Padilla, Eleazar
AU - Castillo-Hernandez, Maria Carmen
N1 - Publisher Copyright:
© 2022 Mashhad University of Medical Sciences. All rights reserved.
PY - 2022/12
Y1 - 2022/12
N2 - Objective(s): Spiders of the Loxosceles genus, known as violin spiders, produce venom with dermonecrotic and systemic effects, as it is a species widely distributed in the world, its study represents a high medical relevance. Systemic loxoscelism, which occurs in 1 in 5 cases and is the most frequent in children, can be fatal, so the study of effective therapy is of great relevance. In the present study, we compared different therapeutic options to mitigate the systemic effects of Loxosceles boneti venom in a model in which prepubertal rats were used. Materials and Methods: A model of systemic intoxication by L. boneti venom was provoked in male Wistar rats. Study groups were formed: healthy control, with venom and untreated control, treatment with N-acetylcysteine, and/or hyperbaric oxygenation therapy. Subsequently, pathological analysis of the kidney and lung was performed. The oxidant-antioxidant response was evaluated, and molecular analysis of the COX-1 and COX-2 enzymes was performed. Results: Regenerative changes were observed at the cellular level in both treatments, being more noticeable in the hyperbaric oxygen therapy (HBO) group. The anti-oxidant response was outstanding in the same group. Conclusion: Both treatments offer considerable benefits, however; further studies are needed to provide adequate therapeutics.
AB - Objective(s): Spiders of the Loxosceles genus, known as violin spiders, produce venom with dermonecrotic and systemic effects, as it is a species widely distributed in the world, its study represents a high medical relevance. Systemic loxoscelism, which occurs in 1 in 5 cases and is the most frequent in children, can be fatal, so the study of effective therapy is of great relevance. In the present study, we compared different therapeutic options to mitigate the systemic effects of Loxosceles boneti venom in a model in which prepubertal rats were used. Materials and Methods: A model of systemic intoxication by L. boneti venom was provoked in male Wistar rats. Study groups were formed: healthy control, with venom and untreated control, treatment with N-acetylcysteine, and/or hyperbaric oxygenation therapy. Subsequently, pathological analysis of the kidney and lung was performed. The oxidant-antioxidant response was evaluated, and molecular analysis of the COX-1 and COX-2 enzymes was performed. Results: Regenerative changes were observed at the cellular level in both treatments, being more noticeable in the hyperbaric oxygen therapy (HBO) group. The anti-oxidant response was outstanding in the same group. Conclusion: Both treatments offer considerable benefits, however; further studies are needed to provide adequate therapeutics.
KW - Anti-oxidant therapy
KW - Cyclooxygenases
KW - Hyperbaric oxygen therapy
KW - Spider venoms
KW - Systemic inflammatory - response
UR - http://www.scopus.com/inward/record.url?scp=85143718092&partnerID=8YFLogxK
U2 - 10.22038/IJBMS.2022.65357.14385
DO - 10.22038/IJBMS.2022.65357.14385
M3 - Artículo
C2 - 36544520
AN - SCOPUS:85143718092
SN - 2008-3866
VL - 25
SP - 1452
EP - 1459
JO - Iranian Journal of Basic Medical Sciences
JF - Iranian Journal of Basic Medical Sciences
IS - 12
ER -