TY - JOUR
T1 - Cometabolic degradation of chlorophenols by a strain of Burkholderia in fed-batch culture
AU - De Los Cobos-Vasconcelos, D.
AU - Santoyo-Tepole, F.
AU - Juárez-Ramírez, C.
AU - Ruiz-Ordaz, N.
AU - Galíndez-Mayer, C. J.J.
PY - 2006/12/6
Y1 - 2006/12/6
N2 - This work deals with the study of mono and dichlorophenols degradation by a strain of Burkholderia isolated from the Santa Alejandrina marsh, Minatitlán, Veracruz, México. This strain also degrades benzene, toluene and xylene and was identified as the N2-fixing bacteria Burkholderia tropicalis. In addition, the cometabolic degradation of 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) was evaluated in batch and fed-batch cultures, using phenol as the primary substrate. This bacterial strain was unable to degrade the highly substituted chlorophenols 2,4,5-TCP, 2,4,6-TCP and pentachlorophenol. Except for 4-CP, the cometabolic degradation of chlorophenols in batch culture was slow and inefficient, but when B. tropicalis was cultivated in fed-batch system, the removal efficiencies obtained were 93% for 2-CP, 95% for 2,6-DCP, 96% for 2,4-DCP and 100% for 4-CP. However, low cell growth yields were obtained in both batch and fed-batch cultures. This could be attributed to the uncoupling action of the aromatic compounds involved and, except for 2,6 DCP, to the accumulation of metabolic by-products.
AB - This work deals with the study of mono and dichlorophenols degradation by a strain of Burkholderia isolated from the Santa Alejandrina marsh, Minatitlán, Veracruz, México. This strain also degrades benzene, toluene and xylene and was identified as the N2-fixing bacteria Burkholderia tropicalis. In addition, the cometabolic degradation of 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) and 2,6-dichlorophenol (2,6-DCP) was evaluated in batch and fed-batch cultures, using phenol as the primary substrate. This bacterial strain was unable to degrade the highly substituted chlorophenols 2,4,5-TCP, 2,4,6-TCP and pentachlorophenol. Except for 4-CP, the cometabolic degradation of chlorophenols in batch culture was slow and inefficient, but when B. tropicalis was cultivated in fed-batch system, the removal efficiencies obtained were 93% for 2-CP, 95% for 2,6-DCP, 96% for 2,4-DCP and 100% for 4-CP. However, low cell growth yields were obtained in both batch and fed-batch cultures. This could be attributed to the uncoupling action of the aromatic compounds involved and, except for 2,6 DCP, to the accumulation of metabolic by-products.
KW - Biodegradation
KW - Biotransformation
KW - Burkholderia tropicalis
KW - Chlorophenol
KW - Cometabolism
KW - Fed-batch
UR - http://www.scopus.com/inward/record.url?scp=33750016610&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2005.10.038
DO - 10.1016/j.enzmictec.2005.10.038
M3 - Artículo
SN - 0141-0229
VL - 40
SP - 57
EP - 60
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 1
ER -