TY - JOUR
T1 - Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition
AU - Jiménez-Guillen, Doribet
AU - Pérez-Pascual, Daniel
AU - Souza-Perera, Ramón
AU - Godoy-Hernández, Gregorio
AU - Zúñiga-Aguilar, José Juan
N1 - Publisher Copyright:
© 2018
PY - 2018/11
Y1 - 2018/11
N2 - Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of somatic embryogenesis can be useful to understand the molecular regulation of the cell-to embryo transition, and these promoter elements represent biotechnological tools in plant organ tissue culture. Results: A − 1,620 bp DNA sequence located upstream of the Coffea canephora SERK1 gene homologue (CcSERK1) was isolated, and then, different segments containing key response elements (REs) for somatic embryogenesis onset and development were fused to the uidA (encoding a β-glucuronidase, GUS) reporter gene to evaluate its expression in transgenic leaf explants. DNA segments of − 1,620 and − 1048 bp in length directed uidA expression with patterns in leaf explants similar to those occurring during somatic embryogenesis. When a − 792-bp fragment was used, uidA expression disappeared only in leaf explants and pro-embryogenic mass but persisted in developing embryos. No uidA expression was detected in any embryogenic stage when a − 618-bp fragment was used. Conclusion: DNA deletions showed that a − 1048-bp sequence located upstream of the CcSERK1 gene is sufficient to direct gene expression during the onset and the development of C. canephora somatic embryogenesis. The DNA segment located between − 1048 and − 792 bp (containing BBM and WUS REs) is needed for gene expression before embryogenesis onset but not during embryo development. The promoter segment between − 792 and − 618 bp (including GATA, ARR1AT, and ANT REs) regulates gene expression in developing embryos. How to cite: Jiménez-Guillen D, Pérez-Pascual D, Souza-Perera R, et al. Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition. Electron J Biotechnol 2018;36.https://doi.org/10.1016/j.ejbt.2018.08.005.
AB - Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis. The identification of promoter elements responsible for SERK1 expression during the onset of somatic embryogenesis can be useful to understand the molecular regulation of the cell-to embryo transition, and these promoter elements represent biotechnological tools in plant organ tissue culture. Results: A − 1,620 bp DNA sequence located upstream of the Coffea canephora SERK1 gene homologue (CcSERK1) was isolated, and then, different segments containing key response elements (REs) for somatic embryogenesis onset and development were fused to the uidA (encoding a β-glucuronidase, GUS) reporter gene to evaluate its expression in transgenic leaf explants. DNA segments of − 1,620 and − 1048 bp in length directed uidA expression with patterns in leaf explants similar to those occurring during somatic embryogenesis. When a − 792-bp fragment was used, uidA expression disappeared only in leaf explants and pro-embryogenic mass but persisted in developing embryos. No uidA expression was detected in any embryogenic stage when a − 618-bp fragment was used. Conclusion: DNA deletions showed that a − 1048-bp sequence located upstream of the CcSERK1 gene is sufficient to direct gene expression during the onset and the development of C. canephora somatic embryogenesis. The DNA segment located between − 1048 and − 792 bp (containing BBM and WUS REs) is needed for gene expression before embryogenesis onset but not during embryo development. The promoter segment between − 792 and − 618 bp (including GATA, ARR1AT, and ANT REs) regulates gene expression in developing embryos. How to cite: Jiménez-Guillen D, Pérez-Pascual D, Souza-Perera R, et al. Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition. Electron J Biotechnol 2018;36.https://doi.org/10.1016/j.ejbt.2018.08.005.
KW - Biotechnological tool
KW - Cell-to-embryo transition
KW - Coffea canephora
KW - Coffee
KW - Gene expression
KW - Plant organ tissue culture
KW - Promoter functional analysis
KW - Reporter genes
KW - Somatic embryogenesis
KW - Transgenic leaf explants
KW - uidA
UR - http://www.scopus.com/inward/record.url?scp=85055199297&partnerID=8YFLogxK
U2 - 10.1016/j.ejbt.2018.08.005
DO - 10.1016/j.ejbt.2018.08.005
M3 - Artículo
AN - SCOPUS:85055199297
SN - 0717-3458
VL - 36
SP - 34
EP - 46
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
ER -