Chitosanase activity in Bacillus thuringiensis

R. Cruz Camarillo, O. Sánchez Pérez, N. G. Rojas Avelizapa, M. Gómez Ramírez, L. I. Rojas Avelizapa

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23 %) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32°C with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
    Original languageAmerican English
    Pages (from-to)94-96
    Number of pages84
    JournalFolia Microbiologica
    DOIs
    StatePublished - 1 Jan 2004

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