TY - JOUR
T1 - Characterization of Potential Virulence Factors of Vibrio mimicus Isolated from Fishery Products and Water
AU - Hernández-Robles, M. F.
AU - Natividad-Bonifacio, I.
AU - Álvarez-Contreras, A. K.
AU - Tercero-Alburo, J. J.
AU - Quinõnes-Ramírez, E. I.
AU - Vázquez-Salinas, C.
N1 - Publisher Copyright:
© 2021 M. F. Hernández-Robles et al.
PY - 2021
Y1 - 2021
N2 - Vibrio mimicus is a Gram-negative bacterium that is closely related to V. cholerae and causes gastroenteritis in humans due to contaminated fish consumption and seafood. This bacterium was isolated and identified from 238 analyzed samples of sea water, oysters, and fish. Twenty strains were identified as V. mimicus according to amplification of the vmhA gene, which is useful as a marker of identification of the species. The production of lipases, proteases, and nucleases was detected; 45% of the strains were able to produce thermonucleases and 40% were capable of producing hydroxamate-type siderophores, and the fragment of the iuT gene was amplified in all of the V. mimicus strains. Seventy-five percent of V. mimicus strains showed cytopathic effect on Chinese hamster ovary (CHO) cells and destruction of the monolayer, and 100% of the strains were adherent on the HEp-2 cell line with an aggregative adherence pattern. The presence of virulence factors in V. mimicus strains obtained from fishery products suggests that another member of the Vibrio genus could represent a risk to the consumer due to production of different metabolites that allows it to subsist in the host.
AB - Vibrio mimicus is a Gram-negative bacterium that is closely related to V. cholerae and causes gastroenteritis in humans due to contaminated fish consumption and seafood. This bacterium was isolated and identified from 238 analyzed samples of sea water, oysters, and fish. Twenty strains were identified as V. mimicus according to amplification of the vmhA gene, which is useful as a marker of identification of the species. The production of lipases, proteases, and nucleases was detected; 45% of the strains were able to produce thermonucleases and 40% were capable of producing hydroxamate-type siderophores, and the fragment of the iuT gene was amplified in all of the V. mimicus strains. Seventy-five percent of V. mimicus strains showed cytopathic effect on Chinese hamster ovary (CHO) cells and destruction of the monolayer, and 100% of the strains were adherent on the HEp-2 cell line with an aggregative adherence pattern. The presence of virulence factors in V. mimicus strains obtained from fishery products suggests that another member of the Vibrio genus could represent a risk to the consumer due to production of different metabolites that allows it to subsist in the host.
UR - http://www.scopus.com/inward/record.url?scp=85101503587&partnerID=8YFLogxK
U2 - 10.1155/2021/8397930
DO - 10.1155/2021/8397930
M3 - Artículo
C2 - 33628259
AN - SCOPUS:85101503587
SN - 1687-918X
VL - 2021
JO - International Journal of Microbiology
JF - International Journal of Microbiology
M1 - 8397930
ER -