TY - JOUR
T1 - Characterization of outer membrane vesicles from Brucella melitensis and protection induced in mice
AU - Avila-Caldern, Eric Daniel
AU - Lopez-Merino, Ahidé
AU - Jain, Neeta
AU - Peralta, Humberto
AU - Lpez-Villegas, Edgar Oliver
AU - Sriranganathan, Nammalwar
AU - Boyle, Stephen M.
AU - Witonsky, Sharon
AU - Contreras-Rodríguez, Araceli
PY - 2012
Y1 - 2012
N2 - The outer membrane vesicles (OMVs) from smooth B. melitensis 16M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. Proteomic analysis showed 29 proteins present in OMVs from B. melitensis 16M; some of them are well-known Brucella immunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16. OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNF, and IFN genes in bone marrow dendritic cells than OMVs from smooth strain 16M. Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strain B. melitensis 16M just as well as the group immunized with live strain B. melitensis Rev1 (P < 0.005). Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.
AB - The outer membrane vesicles (OMVs) from smooth B. melitensis 16M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. Proteomic analysis showed 29 proteins present in OMVs from B. melitensis 16M; some of them are well-known Brucella immunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16. OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNF, and IFN genes in bone marrow dendritic cells than OMVs from smooth strain 16M. Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strain B. melitensis 16M just as well as the group immunized with live strain B. melitensis Rev1 (P < 0.005). Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.
UR - http://www.scopus.com/inward/record.url?scp=84855577119&partnerID=8YFLogxK
U2 - 10.1155/2012/352493
DO - 10.1155/2012/352493
M3 - Artículo
C2 - 22242036
SN - 1740-2522
VL - 2012
JO - Clinical and Developmental Immunology
JF - Clinical and Developmental Immunology
M1 - 352493
ER -