Abstract
A bacterial mixed culture was immobilized in Millipore filters to construct microbial-membranes for BOD determination using an oxygen electrode. The biosensor response was best when 0.5 mg cells were immobilized per cm2 of membrane, at 30°C, pH 7 and 0.05 M phosphate buffer. Reproducible microbial-membranes can be constructed and they can be stored for up to 20 days without appreciable loss of their response characteristics.
Original language | English |
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Pages (from-to) | 399-404 |
Number of pages | 6 |
Journal | Biotechnology Techniques |
Volume | 6 |
Issue number | 5 |
DOIs | |
State | Published - Sep 1992 |
Externally published | Yes |