TY - JOUR
T1 - Characterization of cationic liposomes having IL-2 expressed on their external surface, and their affinity to cervical cancer cells expressing the IL-2 receptor
AU - Corona-Ortega, Teresa
AU - Rangel-Corona, Rosalva
AU - Hernández-Jiménez, Miriam
AU - Baeza, Isabel
AU - Ibáñez, Miguel
AU - Weiss-Steider, Benny
N1 - Funding Information:
Authors acknowledge support from DGAPA, UNAM IN216502, and IN220108 grants. The authors thank Margarita Cruz Millán for assistance in image processing and José Chavarría for excellent technical assistance.
PY - 2009/8
Y1 - 2009/8
N2 - Anionic, cationic, and neutral liposomes were constructed to contain IL-2 in order to evaluate their affinity to a cervical cancer cell line (INBL) and to determine whether they can present IL-2 on their external surface. When these liposomes were co-cultured with INBL, the anionic liposomes were the only ones found to be cytotoxic. When neutral and cationic liposomes were constructed in the presence of IL-2, IL-2 was detected only on the surface of cationic liposomes by using a fluorescent anti-IL-2 antibody. By co-culturing INBL with IL-2-containing cationic liposomes, and by using fluorescent anti-IL-2 antibody, we found a strong IL-2 presence on the cell membranes thus suggesting a high affinity of the liposomes to the INBL cells.
AB - Anionic, cationic, and neutral liposomes were constructed to contain IL-2 in order to evaluate their affinity to a cervical cancer cell line (INBL) and to determine whether they can present IL-2 on their external surface. When these liposomes were co-cultured with INBL, the anionic liposomes were the only ones found to be cytotoxic. When neutral and cationic liposomes were constructed in the presence of IL-2, IL-2 was detected only on the surface of cationic liposomes by using a fluorescent anti-IL-2 antibody. By co-culturing INBL with IL-2-containing cationic liposomes, and by using fluorescent anti-IL-2 antibody, we found a strong IL-2 presence on the cell membranes thus suggesting a high affinity of the liposomes to the INBL cells.
KW - Cationic liposomes
KW - Cervical cancer
KW - IL-2 liposomes
KW - IL-2 receptor
UR - http://www.scopus.com/inward/record.url?scp=70349785540&partnerID=8YFLogxK
U2 - 10.1080/10611860903012810
DO - 10.1080/10611860903012810
M3 - Artículo
C2 - 19514860
SN - 1061-186X
VL - 17
SP - 496
EP - 501
JO - Journal of Drug Targeting
JF - Journal of Drug Targeting
IS - 7
ER -