TY - JOUR
T1 - Changes in vascular reactivity following subrenal aortic constriction in pregnant and nonpregnant rats
AU - Bobadilla, R. A.
AU - Valencia-Hernández, I.
AU - Pérez-Alvarez, V. M.
AU - Mera-Jiménez, E.
AU - Castillo-Henkel, C.
N1 - Funding Information:
We are indebted to Mrs. Lourdes Ramírez Pichardo for technical assistance. This work is supported by a COFAA and CONACYT (México) grant.
PY - 2001
Y1 - 2001
N2 - Objective: The present study was designed to determine whether or not subrenal aortic coarctation (SAC) is able to modify aorta reactivity in pregnant rats. Methods: Wistar female rats were subjected to SAC, and the responses to phenylephrine and acetylcholine of aortic segments above (thoracic) and below (abdominal) the coarctation from pregnant and non-pregnant rats were explored. Results: Contractile responses to phenylephrine and relaxant responses to acetylcholine were similar in the thoracic segment from pregnant and non-pregnant SAC rats, whereas both kinds of response were higher in the abdominal segment from pregnant rats (p < 0.05). L-NAME (a nitric oxide synthase inhibitor) increased the effect of phenylephrine only in the aortic rings from pregnant animals (p < 0.05) and in general abolished the response to acetyl-choline, with the exception of the abdominal segment from pregnant rats, in which only a partial inhibition was observed (p < 0.05). Indomethacin inhibited the contractile response to phenylephrine and increased the relaxant activity to acetylcholine in both aortic segments from the two groups of animals (p < 0.05). Conclusion: The lower contractile response to adrenergic agonists and higher relaxant response to acetylcholine that are associated with normal pregnancy are lost as a consequence of the coarctation procedure. Changes in the production of endothelial nitric oxide and contractile prostanoids appear to be associated with the vascular disturbances observed in SAC rats.
AB - Objective: The present study was designed to determine whether or not subrenal aortic coarctation (SAC) is able to modify aorta reactivity in pregnant rats. Methods: Wistar female rats were subjected to SAC, and the responses to phenylephrine and acetylcholine of aortic segments above (thoracic) and below (abdominal) the coarctation from pregnant and non-pregnant rats were explored. Results: Contractile responses to phenylephrine and relaxant responses to acetylcholine were similar in the thoracic segment from pregnant and non-pregnant SAC rats, whereas both kinds of response were higher in the abdominal segment from pregnant rats (p < 0.05). L-NAME (a nitric oxide synthase inhibitor) increased the effect of phenylephrine only in the aortic rings from pregnant animals (p < 0.05) and in general abolished the response to acetyl-choline, with the exception of the abdominal segment from pregnant rats, in which only a partial inhibition was observed (p < 0.05). Indomethacin inhibited the contractile response to phenylephrine and increased the relaxant activity to acetylcholine in both aortic segments from the two groups of animals (p < 0.05). Conclusion: The lower contractile response to adrenergic agonists and higher relaxant response to acetylcholine that are associated with normal pregnancy are lost as a consequence of the coarctation procedure. Changes in the production of endothelial nitric oxide and contractile prostanoids appear to be associated with the vascular disturbances observed in SAC rats.
KW - Ischemia
KW - Nitric oxide
KW - Pregnancy
KW - Prostaglandins
KW - Rat aorta
UR - http://www.scopus.com/inward/record.url?scp=0034767974&partnerID=8YFLogxK
U2 - 10.1081/PRG-100106964
DO - 10.1081/PRG-100106964
M3 - Artículo
C2 - 12044325
SN - 1064-1955
VL - 20
SP - 143
EP - 156
JO - Hypertension in Pregnancy
JF - Hypertension in Pregnancy
IS - 2
ER -