TY - JOUR
T1 - Cellulomonas flavigena
T2 - Characterization of an endo-1,4-xylanase tightly induced by sugarcane bagasse
AU - Mayorga-Reyes, Lino
AU - Morales, Yolanda
AU - Salgado, Luis M.
AU - Ortega, Arturo
AU - Ponce-Noyola, Teresa
N1 - Funding Information:
This work was supported by CONACYT-México (3528P-B9607 and 28784-B). L.M.-R. was supported by a CONACYT-México Ph.D. scholarship.
PY - 2002/9/10
Y1 - 2002/9/10
N2 - Xylanases, an important group of enzymes for biomass degradation in the industry, are commonly found forming complex multienzyme systems. As a preliminary step to the construction of efficient xylanase producers using genetic engineering, we have characterized a gene encoding an endo-β-1,4 xylanase (xyncflA) from Cellulomonas flavigena. The xylanase activity and the xyncflA synthesis were higher when C. flavigena was grown on sugarcane bagasse. In this substrate, both activity and transcript increased with approximately the same rate during the culture period. When C. flavigena grew on glucose, low signal of mRNA was observed, suggesting that the xyncflA gene is regulated at the transcriptional level.
AB - Xylanases, an important group of enzymes for biomass degradation in the industry, are commonly found forming complex multienzyme systems. As a preliminary step to the construction of efficient xylanase producers using genetic engineering, we have characterized a gene encoding an endo-β-1,4 xylanase (xyncflA) from Cellulomonas flavigena. The xylanase activity and the xyncflA synthesis were higher when C. flavigena was grown on sugarcane bagasse. In this substrate, both activity and transcript increased with approximately the same rate during the culture period. When C. flavigena grew on glucose, low signal of mRNA was observed, suggesting that the xyncflA gene is regulated at the transcriptional level.
KW - Cellulomonas flavigena
KW - Sugarcane bagasse
KW - Transcriptional regulation
KW - Xylanase
UR - http://www.scopus.com/inward/record.url?scp=0037057134&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(02)00876-5
DO - 10.1016/S0378-1097(02)00876-5
M3 - Artículo
SN - 0378-1097
VL - 214
SP - 205
EP - 209
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -