Cell yield and superoxide dismutase activity of the marine yeast Debaryomyces hansenii under different culture conditions

Martin Ramírez Orozco, Norma Y. Hernández-Saavedra, Felipe Ascencio Valle, Bárbara Acosta González, José Luis Ochoa

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The effect of aeration, pH, stirring rate, and temperature on the biomass production and superoxide dismutase (SOD) activity of the marine yeast Debaryomyces hansenii strain C-11 was determined. The cell biomass yield was approximately 50% in a seawater-formulated medium using glucose as the carbon source. The SOD activity increased by application of a pulse of oxygen or 0.8 mM sulfate copper into the chemical reactor. The SOD enzyme had an activity of 400 units/mg of protein in a crude extract produced under such conditions, the best activity ever reported for this enzyme in a crude preparation.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalJournal of Marine Biotechnology
Volume6
Issue number4
StatePublished - 1998
Externally publishedYes

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