TY - JOUR
T1 - CCL28 Is Involved in Mucosal IgA Responses, Olfaction, and Resistance to Enteric Infections
AU - Burkhardt, Amanda M.
AU - Perez-Lopez, Araceli
AU - Ushach, Irina
AU - Catalan-Dibene, Jovani
AU - Nuccio, Sean Paul
AU - Chung, Lawton K.
AU - Hernandez-Ruiz, Marcela
AU - Carnevale, Christina
AU - Raffatellu, Manuela
AU - Zlotnik, Albert
N1 - Publisher Copyright:
© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.
PY - 2019/4
Y1 - 2019/4
N2 - CCL28 is a mucosal chemokine that has been involved in various responses, including IgA production. We have analyzed its production in human tissues using a comprehensive microarray database. Its highest expression is in the salivary gland, indicating that it is an important component of saliva. It is also expressed in the trachea, bronchus, and in the mammary gland upon onset of lactation. We have also characterized a Ccl28 -/- mouse that exhibits very low IgA levels in milk, and the IgA levels in feces are also reduced. These observations confirm a role for the CCL28/CCR10 chemokine axis in the recruitment of IgA plasmablasts to the lactating mammary gland. CCL28 is also expressed in the vomeronasal organ. We also detected olfactory defects (anosmia) in a Ccl28 -/- mouse suggesting that CCL28 is involved in the function/development of olfaction. Importantly, Ccl28 -/- mice are highly susceptible to Salmonella enterica serovar Typhimurium in an acute model of infection, indicating that CCL28 plays a major role in innate immunity against Salmonella in the gut. Finally, microbiome studies revealed modest differences in the gut microbiota between Ccl28 -/- mice and their cohoused wild-type littermates. The latter observation suggests that under homeostatic conditions, CCL28 plays a limited role in shaping the gut microbiome.
AB - CCL28 is a mucosal chemokine that has been involved in various responses, including IgA production. We have analyzed its production in human tissues using a comprehensive microarray database. Its highest expression is in the salivary gland, indicating that it is an important component of saliva. It is also expressed in the trachea, bronchus, and in the mammary gland upon onset of lactation. We have also characterized a Ccl28 -/- mouse that exhibits very low IgA levels in milk, and the IgA levels in feces are also reduced. These observations confirm a role for the CCL28/CCR10 chemokine axis in the recruitment of IgA plasmablasts to the lactating mammary gland. CCL28 is also expressed in the vomeronasal organ. We also detected olfactory defects (anosmia) in a Ccl28 -/- mouse suggesting that CCL28 is involved in the function/development of olfaction. Importantly, Ccl28 -/- mice are highly susceptible to Salmonella enterica serovar Typhimurium in an acute model of infection, indicating that CCL28 plays a major role in innate immunity against Salmonella in the gut. Finally, microbiome studies revealed modest differences in the gut microbiota between Ccl28 -/- mice and their cohoused wild-type littermates. The latter observation suggests that under homeostatic conditions, CCL28 plays a limited role in shaping the gut microbiome.
KW - Salmonella
KW - chemokines
KW - gut
KW - innate immunity
KW - vomeronasal organ
UR - http://www.scopus.com/inward/record.url?scp=85064076681&partnerID=8YFLogxK
U2 - 10.1089/jir.2018.0099
DO - 10.1089/jir.2018.0099
M3 - Artículo
C2 - 30855201
AN - SCOPUS:85064076681
SN - 1079-9907
VL - 39
SP - 214
EP - 223
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 4
ER -