TY - JOUR
T1 - Carotenogenic gene expression and ultrastructural changes during development in marigold
AU - Del Villar-Martínez, Alma A.
AU - García-Saucedo, Pedro A.
AU - Carabez-Trejo, Alfonso
AU - Cruz-Hernández, Andrés
AU - Paredes-López, Octavio
N1 - Funding Information:
We would like to thank Prof. F. X. Cunningham Jr. from the University of Maryland, Prof. D. Grierson from University of Nottingham, England, and Glenn E. Bartley from DuPont Central Research and Development, Wilmington, Delaware, for kindly providing us with the cDNAs used as heterologous probes; to Mr. J.L. Hernández-Flores, CINVESTAV, and Mrs. Lourdes Palma-Tirado, UNAM, for help with the sequence and TEM analyses respectively; and to F. Guevara and M.E. Valverde for reviewing the manuscript. This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) and Sistema de Investigación Miguel Hidalgo (SIHGO), México. DVMAA would like to thank CONACYT and COFAA for Ph.D. scholarships.
PY - 2005/9/14
Y1 - 2005/9/14
N2 - Marigold (Tagetes erecta) flowers are a good source of carotenoids and can be used as a model studies on pigmentation during flower development. They show different levels of pigmentation caused by lutein. Here we describe the expression of several genes in the carotenoid biosynthetic pathway: phytoene synthase (Psy), phytoene desaturase (Pds), lycopene β-cyclase (Lcy-b) and lycopene ε-cyclase (Lcy-e). cDNA inserts from isolated clones were 1376-1916 bp long. The predicted amino acid sequences showed from 66 to 100% homology with other reported sequences (NCBI gene bank). Northern blot analyses of three varieties of marigold showed that most gene transcripts were expressed during flower development. The ultrastructural changes that occurred during plastid differentiation in flower morphogenesis were analyzed, and pigment accumulation among varieties was evaluated. The pigment deposition in specific structures (lipidic vesicles) during flower development was demonstrated.
AB - Marigold (Tagetes erecta) flowers are a good source of carotenoids and can be used as a model studies on pigmentation during flower development. They show different levels of pigmentation caused by lutein. Here we describe the expression of several genes in the carotenoid biosynthetic pathway: phytoene synthase (Psy), phytoene desaturase (Pds), lycopene β-cyclase (Lcy-b) and lycopene ε-cyclase (Lcy-e). cDNA inserts from isolated clones were 1376-1916 bp long. The predicted amino acid sequences showed from 66 to 100% homology with other reported sequences (NCBI gene bank). Northern blot analyses of three varieties of marigold showed that most gene transcripts were expressed during flower development. The ultrastructural changes that occurred during plastid differentiation in flower morphogenesis were analyzed, and pigment accumulation among varieties was evaluated. The pigment deposition in specific structures (lipidic vesicles) during flower development was demonstrated.
KW - Asteraceae
KW - Carotenoids
KW - Chromoplasts
KW - Lutein
KW - Marigold
KW - Tagetes erecta
UR - http://www.scopus.com/inward/record.url?scp=23844537514&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2004.12.004
DO - 10.1016/j.jplph.2004.12.004
M3 - Artículo
SN - 0176-1617
VL - 162
SP - 1046
EP - 1056
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
IS - 9
ER -