TY - JOUR
T1 - Capillary electrophoresis separation of monoclonal antibody isoforms using a neutral capillary
AU - Espinosa-De La Garza, Carlos Eduardo
AU - Salazar-Flores, Rodolfo Daniel
AU - Pérez, Néstor Octavio
AU - Flores-Ortiz, Luis Francisco
AU - Medina-Rivero, Emilio
N1 - Publisher Copyright:
© 2017 Journal of Visualized Experiments.
PY - 2017/1/16
Y1 - 2017/1/16
N2 - Biotherapeutic proteins, such as monoclonal antibodies (mAbs), are feasible alternatives for the treatment of chronic-degenerative diseases. The biological activity of these proteins depends on their physicochemical properties. The use of high-performance techniques like chromatography and capillary electrophoresis has been described for the analysis of physicochemical heterogeneity of mAbs. Nowadays, capillary zone electrophoresis (CZE) technique constitutes one of the most resolutive and sensitive assays for the analysis of biomolecules. Besides, the electro-driven separation in CZE is governed by extensive properties of matter and offers the advantage of analyzing proteins close to their native state. However, the successful implementation of this technique for routine analysis depends on the skills of the analyst at the critical steps during sample and system preparation. The purpose of this tutorial is to detail the steps to succeed in the CZE analysis of mAbs. Further, this protocol can be used for the development and improvement of skills of the personnel involved in protein analytical chemistry laboratories.
AB - Biotherapeutic proteins, such as monoclonal antibodies (mAbs), are feasible alternatives for the treatment of chronic-degenerative diseases. The biological activity of these proteins depends on their physicochemical properties. The use of high-performance techniques like chromatography and capillary electrophoresis has been described for the analysis of physicochemical heterogeneity of mAbs. Nowadays, capillary zone electrophoresis (CZE) technique constitutes one of the most resolutive and sensitive assays for the analysis of biomolecules. Besides, the electro-driven separation in CZE is governed by extensive properties of matter and offers the advantage of analyzing proteins close to their native state. However, the successful implementation of this technique for routine analysis depends on the skills of the analyst at the critical steps during sample and system preparation. The purpose of this tutorial is to detail the steps to succeed in the CZE analysis of mAbs. Further, this protocol can be used for the development and improvement of skills of the personnel involved in protein analytical chemistry laboratories.
KW - Biochemistry
KW - Capillary electrophoresis
KW - Characterization
KW - Issue 119
KW - Monoclonal antibodies
KW - Neutral capillary
KW - Physicochemical heterogeneity
KW - Troubleshooting
UR - http://www.scopus.com/inward/record.url?scp=85011590988&partnerID=8YFLogxK
U2 - 10.3791/55082
DO - 10.3791/55082
M3 - Artículo
C2 - 28117815
SN - 1940-087X
VL - 2017
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 119
M1 - e55082
ER -