TY - JOUR
T1 - Calidad de la proteína en líneas e híbridos experimentales de QPM. Altiplano Central, México
AU - Arellano-Vázquez, José Luis
AU - Gutiérrez-Hernández, Germán Fernando
AU - Flores-Gómez, Estela
AU - López-Martínez, Daniel Eduardo
N1 - Publisher Copyright:
© 2021 Polish Academy of Sciences. All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Introduction. Currently, maize is the most cultivated grain in the world and it is feasible to improve the nutrition of its consumers through the development of quality protein maize (QPM), whose basis is the expression of the opaque2 gene, which increases the contents of lysine and tryptophan in the grain. Objective. To analyze chemically the protein of the grain of lines and experimental simple hybrids of QPM and to know the relationships between the genotype and the evaluated variables of protein quality. Materials and methods. The experiments were carried out during 2017 in the Interdisciplinary Professional Unit of Biotechnology of the Instituto Politecnico Nacional (Mexico City). Whole grains of parental lines (P1 to P6) and direct simple hybrids were used. Lysine, tryptophan, and protein quantity and quality were estimated. A completely randomized design with three replications was used. Analysis of variance, comparison of means, and principal component analysis were performed. Results. Lines P2, P3, and P6, and hybrids P2 x P4 and P2 x P6 were identified as significant (p ≤ 0.05) QPM. The protein quality index was correlated with tryptophan (r = 0.9 *) and with lysine (r = 0.7 *) but not with total protein. Conclusions. The fundamental factor for developing the QPM were the essential amino acids and the limiting factor for increasing their nutritional value was the quality of the protein.
AB - Introduction. Currently, maize is the most cultivated grain in the world and it is feasible to improve the nutrition of its consumers through the development of quality protein maize (QPM), whose basis is the expression of the opaque2 gene, which increases the contents of lysine and tryptophan in the grain. Objective. To analyze chemically the protein of the grain of lines and experimental simple hybrids of QPM and to know the relationships between the genotype and the evaluated variables of protein quality. Materials and methods. The experiments were carried out during 2017 in the Interdisciplinary Professional Unit of Biotechnology of the Instituto Politecnico Nacional (Mexico City). Whole grains of parental lines (P1 to P6) and direct simple hybrids were used. Lysine, tryptophan, and protein quantity and quality were estimated. A completely randomized design with three replications was used. Analysis of variance, comparison of means, and principal component analysis were performed. Results. Lines P2, P3, and P6, and hybrids P2 x P4 and P2 x P6 were identified as significant (p ≤ 0.05) QPM. The protein quality index was correlated with tryptophan (r = 0.9 *) and with lysine (r = 0.7 *) but not with total protein. Conclusions. The fundamental factor for developing the QPM were the essential amino acids and the limiting factor for increasing their nutritional value was the quality of the protein.
KW - Biofortification
KW - Essential amino acids
KW - Opaque2
KW - Protein quality
UR - http://www.scopus.com/inward/record.url?scp=85120090476&partnerID=8YFLogxK
U2 - 10.15517/am.v32i1.40439
DO - 10.15517/am.v32i1.40439
M3 - Artículo
AN - SCOPUS:85120090476
SN - 2215-3608
VL - 32
SP - 107
EP - 119
JO - Agronomy Mesoamerican
JF - Agronomy Mesoamerican
IS - 1
ER -