Abstract
Intestinal intraepithelial lymphocytes (IEL) do not proliferate in response to polyclonal mitogens and TCR/CD3-mediated stimulation to the same extent as other lymphocyte populations in mammals or birds. Increases in intracellular free Ca2+ and tyrosine phosphorylation of a limited number of cellular substrates are recognized as key biochemical events following stimulation of mature T cells via TCR/CD3. We are therefore investigating the possibility that signal transduction pathways are differentially regulated in both avian and mammalian intestinal IEL. We have observed that Ca2+ mobilization in response to ionomycin treatment and to CD3 crosslinking is impaired in chicken IEL as compared to splenic lymphocytes. Western blot analyses reveal different patterns of tyrosine-phosphorylated proteins in intestinal IEL, as compared to thymic and splenic T cells. Similar studies are underway using mouse intestinal IEL and immunoprecipitation experiments are being performed to identify candidate tyrosine-phosphorylated proteins in intestinal IEL. Alterations in intracellular signalling events in intestinal IEL reveal additional properties of IEL that may be unique to their tissue microenvironment and/or function.
| Original language | English |
|---|---|
| Pages (from-to) | A1448 |
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| State | Published - 1996 |
| Externally published | Yes |