TY - JOUR
T1 - Broth rheology, growth and metabolite production of Beta vulgaris suspension culture
T2 - A comparative study between cultures grown in shake flasks and in a stirred tank
AU - Rodríguez-Monroy, Mario
AU - Galindo, Enrique
N1 - Funding Information:
This work was partially financed by DGAPA/UNAM (Grant No. IN-503295), and CONACyT (Grant No. 25165-B and 26405-N). M. Rodrı́guez-Monroy is indebted to COFAA-IPN and CONACyT for the doctoral fellowship awarded. We thank Dra. Gladys Casaab, Edith Garcı́a and Gabriela Sepúlveda for assisting in extrallular compounds analyses. Helpful discussions with Drs. O. T. Ramı́rez, M. Rocha, A. López-Munguı́a, L. Serrano and C. Peña are acknowledged with thanks.
PY - 1999/7/1
Y1 - 1999/7/1
N2 - Cells of Beta vulgaris have the ability to grow in a stirred tank under an impeller tip speed as high as 95.3 cm seg-1. Comparing this system with cultures performing in shake flasks, a decrease of the cell concentration, betalains production, and growth rate was observed. However, the kinetic profiles of aggregates size and cellular viability were practically the same. The cultures carried out in the fermentor showed a major accumulation of extracellular arabinogalactoprotein and polysaccharide, which is an indication of the cell response to hydrodynamic stress. These extracellular molecules produced a considerable change in the rheology of cell-free medium. This change in the rheology can be playing an important role in the reduction of the actual hydrodynamic stress during cultivation. Copyright (C) 1999 Elsevier Science Inc. All rights reserved.
AB - Cells of Beta vulgaris have the ability to grow in a stirred tank under an impeller tip speed as high as 95.3 cm seg-1. Comparing this system with cultures performing in shake flasks, a decrease of the cell concentration, betalains production, and growth rate was observed. However, the kinetic profiles of aggregates size and cellular viability were practically the same. The cultures carried out in the fermentor showed a major accumulation of extracellular arabinogalactoprotein and polysaccharide, which is an indication of the cell response to hydrodynamic stress. These extracellular molecules produced a considerable change in the rheology of cell-free medium. This change in the rheology can be playing an important role in the reduction of the actual hydrodynamic stress during cultivation. Copyright (C) 1999 Elsevier Science Inc. All rights reserved.
KW - Arabinogalactoprotein
KW - Betalains
KW - Hydrodynamic stress
KW - Rheology
UR - http://www.scopus.com/inward/record.url?scp=0344483911&partnerID=8YFLogxK
U2 - 10.1016/S0141-0229(99)00002-2
DO - 10.1016/S0141-0229(99)00002-2
M3 - Artículo
SN - 0141-0229
VL - 24
SP - 687
EP - 693
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 10
ER -