TY - JOUR
T1 - Blood SC5b-9 complement levels increase at parturition during term and preterm labor
AU - Segura-Cervantes, Enrique
AU - Mancilla-Ramirez, Javier
AU - Zurita, Luis
AU - Paredes, Yuriria
AU - Arredondo, José Luis
AU - Galindo-Sevilla, Norma
N1 - Publisher Copyright:
© 2015 Elsevier Ireland Ltd.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - We explored the hypothesis that complement, an innate and adaptive immune effector, is active in the plasma of parturient women and is deposited on fetal membranes collected after delivery. A cross-sectional study was designed to evaluate complement activity at parturition. Pregnant women (n = 97) between 15 and 41 years of age were enrolled in a hospital protocol during the perinatal period to assess both SC5b-9 complement activity in blood and complement deposition on fetal membranes during parturition. Soluble SC5b-9 complement activity in plasma fractions was measured using a standard enzyme-linked immunosorbent assay (ELISA) that included specific anti-complement antibodies. Complement deposition on membranes was analyzed using immuno-dot blots and immunohistochemistry. Soluble SC5b-9 complement complex levels were increased in the plasma of women during term labor (TL; median 3361; range 1726-5670. ng/mL), preterm labor (PL; median 2958; range 1552-7092. ng/mL), and preterm premature rupture of membranes (PPROM; median 2272; range 167-6540. ng/mL) compared with pregnant women who were not in labor (P; median 1384; range 174-4570. ng/mL; P < 0.001, Kruskal-Wallis test). Active complement, as assessed by the C9 neo-antigen in C5b-9 complexes, was deposited on fetal membranes, with no difference between term and preterm delivery. The deposition of active complement on fetal membranes was confirmed by immunohistochemistry. Women who underwent non-labor-indicated Cesarean sections did not exhibit complement deposition. Soluble SC5b-9 complement complex levels increased in the plasma of women during parturition, and complement C5b-9 complexes were deposited on fetal membranes.
AB - We explored the hypothesis that complement, an innate and adaptive immune effector, is active in the plasma of parturient women and is deposited on fetal membranes collected after delivery. A cross-sectional study was designed to evaluate complement activity at parturition. Pregnant women (n = 97) between 15 and 41 years of age were enrolled in a hospital protocol during the perinatal period to assess both SC5b-9 complement activity in blood and complement deposition on fetal membranes during parturition. Soluble SC5b-9 complement activity in plasma fractions was measured using a standard enzyme-linked immunosorbent assay (ELISA) that included specific anti-complement antibodies. Complement deposition on membranes was analyzed using immuno-dot blots and immunohistochemistry. Soluble SC5b-9 complement complex levels were increased in the plasma of women during term labor (TL; median 3361; range 1726-5670. ng/mL), preterm labor (PL; median 2958; range 1552-7092. ng/mL), and preterm premature rupture of membranes (PPROM; median 2272; range 167-6540. ng/mL) compared with pregnant women who were not in labor (P; median 1384; range 174-4570. ng/mL; P < 0.001, Kruskal-Wallis test). Active complement, as assessed by the C9 neo-antigen in C5b-9 complexes, was deposited on fetal membranes, with no difference between term and preterm delivery. The deposition of active complement on fetal membranes was confirmed by immunohistochemistry. Women who underwent non-labor-indicated Cesarean sections did not exhibit complement deposition. Soluble SC5b-9 complement complex levels increased in the plasma of women during parturition, and complement C5b-9 complexes were deposited on fetal membranes.
KW - Complement
KW - Fetal membranes
KW - Labor
KW - Parturition
KW - Pregnancy
UR - http://www.scopus.com/inward/record.url?scp=84928768143&partnerID=8YFLogxK
U2 - 10.1016/j.jri.2015.02.008
DO - 10.1016/j.jri.2015.02.008
M3 - Artículo
C2 - 25868739
SN - 0165-0378
VL - 109
SP - 24
EP - 30
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
ER -