TY - JOUR
T1 - Biosensor of inflammation biomarkers based on electrical bioimpedance analysis on immobilized DNA without chemical modification
AU - Gómez-López, Modesto
AU - Miliar-García, Ángel
AU - Pérez-Vielma, Nadia Mabel
AU - Lara-Padilla, Eleazar
AU - González-Díaz, César Antonio
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020
Y1 - 2020
N2 - The development of biosensors to identify molecular markers or specific genes is fundamental for the implementation of new techniques that allow the detection of specific Deoxyribonucleic acid (DNA) sequences in a fast, economic and simple way. Different detection techniques have been proposed in the development of biosensors. Electrical Bioimpedance Spectroscopy (EBiS) has been used for diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and DNA´s immobilization with no chemical modifications. The evaluation of its potential utility in the detection of the gene expression of three inflammation characteristic biomarkers (NLRP3, IL-1β and Caspase 1) is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, measurements that were validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility for a biosensor of specific genes through bioimpedance measurements on the immobilization of cDNA.
AB - The development of biosensors to identify molecular markers or specific genes is fundamental for the implementation of new techniques that allow the detection of specific Deoxyribonucleic acid (DNA) sequences in a fast, economic and simple way. Different detection techniques have been proposed in the development of biosensors. Electrical Bioimpedance Spectroscopy (EBiS) has been used for diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and DNA´s immobilization with no chemical modifications. The evaluation of its potential utility in the detection of the gene expression of three inflammation characteristic biomarkers (NLRP3, IL-1β and Caspase 1) is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, measurements that were validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility for a biosensor of specific genes through bioimpedance measurements on the immobilization of cDNA.
KW - Bioimpedance
KW - Biosensor
KW - DNA
KW - Gene
UR - http://www.scopus.com/inward/record.url?scp=85091510535&partnerID=8YFLogxK
U2 - 10.2478/JOEB-2020-0006
DO - 10.2478/JOEB-2020-0006
M3 - Artículo
AN - SCOPUS:85091510535
SN - 1891-5469
VL - 11
SP - 31
EP - 37
JO - Journal of Electrical Bioimpedance
JF - Journal of Electrical Bioimpedance
IS - 1
ER -