TY - JOUR
T1 - Bioremediation of soils from oil spill impacted sites using bioaugmentation with biosurfactants producing, native, free-living nitrogen fixing bacteria
AU - Pérez Vargas, Josefina
AU - Vigueras Carmona, Sergio Esteban
AU - Zamudio Moreno, Enid
AU - Rivera Casado, Noemí Araceli
AU - Calva Calva, Graciano
N1 - Publisher Copyright:
© 2017, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Oil spills are the most common source of environmental damage in areas with oil extraction activities. Bioaugmentation is a bioremediation technology that involves increasing microbiotas that can remove contaminant compounds from soils or water. This can be achieved by supplying exogenous microorganisms or by increasing the abundance of native microorganisms able to remove the contaminants. As biosurfactants enhance the solubility and bioavailability of hydrophobic compounds, the addition of native bacteria that produce biosurfactants may improve the rates of hydrocarbons biodegradation at oil spill impacted sites. The objective of this study was to investigate the effectiveness of using the bioaugmentation of native, free-living nitrogen fixing bacteria (NFB) that are able to remove hydrocarbons and produce biosurfactant compounds, to bioremediate soils at long term oil spill impacted sites containing total hydrocarbon (TH) levels of 120 000 ppm. For this study, two NFB that produce biosurfactants were selected from a group of 24 previously isolated from a long-term oil spill impacted site. After 16 months of bioaugmentation, the hydrocarbons removal reached 80%. The NFB count increased from 13×104 colony-forming units (CFU) to 2×109 CFU, and the total bacterial population increased from 5×104 CFU to 22×1011 CFU. According to literature, this is the first bioaugmentation study using native NFB biosurfactant producers to bioremediate soils from aged oil spill impacted sites. As such, these results demonstrate the potential of this bioaugmentation strategy for the bioremediation of oil spill impacted sites, especially those low in nitrogen.
AB - Oil spills are the most common source of environmental damage in areas with oil extraction activities. Bioaugmentation is a bioremediation technology that involves increasing microbiotas that can remove contaminant compounds from soils or water. This can be achieved by supplying exogenous microorganisms or by increasing the abundance of native microorganisms able to remove the contaminants. As biosurfactants enhance the solubility and bioavailability of hydrophobic compounds, the addition of native bacteria that produce biosurfactants may improve the rates of hydrocarbons biodegradation at oil spill impacted sites. The objective of this study was to investigate the effectiveness of using the bioaugmentation of native, free-living nitrogen fixing bacteria (NFB) that are able to remove hydrocarbons and produce biosurfactant compounds, to bioremediate soils at long term oil spill impacted sites containing total hydrocarbon (TH) levels of 120 000 ppm. For this study, two NFB that produce biosurfactants were selected from a group of 24 previously isolated from a long-term oil spill impacted site. After 16 months of bioaugmentation, the hydrocarbons removal reached 80%. The NFB count increased from 13×104 colony-forming units (CFU) to 2×109 CFU, and the total bacterial population increased from 5×104 CFU to 22×1011 CFU. According to literature, this is the first bioaugmentation study using native NFB biosurfactant producers to bioremediate soils from aged oil spill impacted sites. As such, these results demonstrate the potential of this bioaugmentation strategy for the bioremediation of oil spill impacted sites, especially those low in nitrogen.
KW - Aged sites
KW - Biopile
KW - Emulsification index
KW - NFB
KW - Rhamnolipid
UR - http://www.scopus.com/inward/record.url?scp=85017403020&partnerID=8YFLogxK
U2 - 10.20937/RICA.2017.33.esp01.09
DO - 10.20937/RICA.2017.33.esp01.09
M3 - Artículo
SN - 0188-4999
VL - 33
SP - 105
EP - 114
JO - Revista Internacional de Contaminacion Ambiental
JF - Revista Internacional de Contaminacion Ambiental
ER -