TY - JOUR
T1 - Bioprofiling of Mexican Plectranthus amboinicus (Lour.) essential oil via planar chromatography–effect-directed analysis combined with direct analysis in real time high-resolution mass spectrometry
AU - Bañuelos-Hernández, A. E.
AU - Azadniya, E.
AU - Ramírez Moreno, E.
AU - Morlock, G. E.
N1 - Publisher Copyright:
© 2020, © 2020 Taylor & Francis Group, LLC.
PY - 2020/6/14
Y1 - 2020/6/14
N2 - Aerial parts of Plectranthus amboinicus (Lour.) were collected in the Northern Mexico City for a comprehensive effect-directed profiling. Its hydrodistilled P. amboinicus essential oil (PAEO) was separated on HPTLC silica gel plates with n-hexane—ethyl acetate—ethanol, 95:3:2, followed by derivatization with the anisaldehyde–sulfuric acid reagent. The UV/Vis/FLD detection was expanded by a biological and biochemical detection. Eight different effect-directed assays were performed including the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay as well as biochemical assays for inhibition of acetylcholinesterase, α- and β-glucosidases, α-amylase and tyrosinase as well as antimicrobial assays against Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bacteria. The bioprofiling, subsequent direct analysis in real time mass spectrometry of the detected five bioactive compound zones and comparison with literature data resulted in the tentative assignment of caryophyllene oxide (hRF 20), α-humulene (hRF 26), carvacrol (hRF 40), methyl carvacrol ether (hRF 76) and caryophyllene (hRF 84). The antimicrobial activity against Gram-negative bacteria of PAEO was elicited by a mixture of the multi-potent compounds, whereas α-humulene was strongly acting against Gram-positive bacteria. The antioxidant effect was related to carvacrol and the inhibitory effect over AChE, tyrosinase and glucosidases to α-humulene.
AB - Aerial parts of Plectranthus amboinicus (Lour.) were collected in the Northern Mexico City for a comprehensive effect-directed profiling. Its hydrodistilled P. amboinicus essential oil (PAEO) was separated on HPTLC silica gel plates with n-hexane—ethyl acetate—ethanol, 95:3:2, followed by derivatization with the anisaldehyde–sulfuric acid reagent. The UV/Vis/FLD detection was expanded by a biological and biochemical detection. Eight different effect-directed assays were performed including the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay as well as biochemical assays for inhibition of acetylcholinesterase, α- and β-glucosidases, α-amylase and tyrosinase as well as antimicrobial assays against Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bacteria. The bioprofiling, subsequent direct analysis in real time mass spectrometry of the detected five bioactive compound zones and comparison with literature data resulted in the tentative assignment of caryophyllene oxide (hRF 20), α-humulene (hRF 26), carvacrol (hRF 40), methyl carvacrol ether (hRF 76) and caryophyllene (hRF 84). The antimicrobial activity against Gram-negative bacteria of PAEO was elicited by a mixture of the multi-potent compounds, whereas α-humulene was strongly acting against Gram-positive bacteria. The antioxidant effect was related to carvacrol and the inhibitory effect over AChE, tyrosinase and glucosidases to α-humulene.
KW - Antimicrobial assay
KW - Plectranthus amboinicus essential oil
KW - bioactive compounds
KW - direct analysis in real time high-resolution mass spectrometry (DART-HRMS)
KW - enzyme assay
UR - http://www.scopus.com/inward/record.url?scp=85081737073&partnerID=8YFLogxK
U2 - 10.1080/10826076.2020.1737542
DO - 10.1080/10826076.2020.1737542
M3 - Artículo
AN - SCOPUS:85081737073
SN - 1082-6076
VL - 43
SP - 344
EP - 350
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 9-10
ER -