TY - JOUR
T1 - Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae
AU - Tamayo-Ordoñez, Yahaira de Jesús
AU - Ayil-Gutiérrez, Benjamín Abraham
AU - Moreno-Davila, Ileana Mayela Maria
AU - Tamayo-Ordoñez, Francisco Alberto
AU - Córdova-Quiroz, Atl Victor
AU - Poot-Poot, Wilberth Alfredo
AU - Damas-Damas, Siprian
AU - Villanueva-Alonzo, Hernán de Jesús
AU - Tamayo-Ordoñez, Maria Concepción
N1 - Publisher Copyright:
© 2022 The Authors. Phycological Research published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Phycology.
PY - 2023/1
Y1 - 2023/1
N2 - Microalgae have been positioned as excellent models for producing new sources of energy (biofuels and biohydrogen). Some investigations in these biological models have been directed to know if the enzymes ferredoxin (FDX) and hydrogenase (HYD) are involved in the algae producing different concentrations of molecular hydrogen (H2). To date, little is known about the concomitant transcriptional regulation of both enzymes during H2 evolution in algae. In this research, we evaluated the relative expression of hdy and fdx genes during the evolution of H2 in three microalgae (Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii) in N-deprived anaerobic cultures in the presence of Fe, and 12:24 and 24:24 h dark:light cycles. We also detected structural differences in the enzymes. The 3D modeling indicated that the 3D structure of HYD and FDX are conserved in most algal genera, and the results of our grouping according to the aa characteristics of the proteins showed two grouping trends: One, according to the algae's phylogenetic classification, and another one according to the species-specific enzyme's characteristics, and the grouping could perhaps be more influenced by the algae's ability to produce H2. The three microalgae species reached maximum H2 accumulation values in 24h:24 h dark:light conditions in Fe-supplemented media (4.2 ± 0.12 mL L−1 in C. vulgaris, 3.9 ± 0.10 mL L−1 in S. obliquus, and 4.5 ± 0.10 mL L−1 in C. reinhardtii), and the highest global relative expression of hyd and fdx genes was reached during the first hour of exposure to light, which suggests concomitant expression of both enzymes at the beginning of H2 production. The behavior of the expression of the hyd and fdx genes in these algal species proved to be similar between species. A better understanding of the concomitant regulation of both enzymes could lay the groundwork for the future use of both enzymes to improve H2 yields in microalgae.
AB - Microalgae have been positioned as excellent models for producing new sources of energy (biofuels and biohydrogen). Some investigations in these biological models have been directed to know if the enzymes ferredoxin (FDX) and hydrogenase (HYD) are involved in the algae producing different concentrations of molecular hydrogen (H2). To date, little is known about the concomitant transcriptional regulation of both enzymes during H2 evolution in algae. In this research, we evaluated the relative expression of hdy and fdx genes during the evolution of H2 in three microalgae (Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii) in N-deprived anaerobic cultures in the presence of Fe, and 12:24 and 24:24 h dark:light cycles. We also detected structural differences in the enzymes. The 3D modeling indicated that the 3D structure of HYD and FDX are conserved in most algal genera, and the results of our grouping according to the aa characteristics of the proteins showed two grouping trends: One, according to the algae's phylogenetic classification, and another one according to the species-specific enzyme's characteristics, and the grouping could perhaps be more influenced by the algae's ability to produce H2. The three microalgae species reached maximum H2 accumulation values in 24h:24 h dark:light conditions in Fe-supplemented media (4.2 ± 0.12 mL L−1 in C. vulgaris, 3.9 ± 0.10 mL L−1 in S. obliquus, and 4.5 ± 0.10 mL L−1 in C. reinhardtii), and the highest global relative expression of hyd and fdx genes was reached during the first hour of exposure to light, which suggests concomitant expression of both enzymes at the beginning of H2 production. The behavior of the expression of the hyd and fdx genes in these algal species proved to be similar between species. A better understanding of the concomitant regulation of both enzymes could lay the groundwork for the future use of both enzymes to improve H2 yields in microalgae.
KW - H production
KW - ferredoxin gene
KW - hydrogenase gene
KW - microalgae
KW - mutation
UR - http://www.scopus.com/inward/record.url?scp=85138338222&partnerID=8YFLogxK
U2 - 10.1111/pre.12500
DO - 10.1111/pre.12500
M3 - Artículo
AN - SCOPUS:85138338222
SN - 1322-0829
VL - 71
SP - 37
EP - 55
JO - Phycological Research
JF - Phycological Research
IS - 1
ER -