TY - JOUR
T1 - Bioimpedance spectroscopy as a potential technique to detect label-free PCR products
AU - Ames-Lastra, G.
AU - Sánchez, V.
AU - Sacristán-Rock, E.
AU - Gómez-López, M.
AU - Pérez-Vielma, N.
AU - Castillo-Salazar, I. A.
AU - Hernández-Nava, A.
AU - González-Díaz, C. A.
N1 - Publisher Copyright:
© Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence.
PY - 2021/11/15
Y1 - 2021/11/15
N2 - PCR is a molecular technique that multiplies DNA fragments in a logarithmical way. qPCR uses fluoroscopic dyes or probes to quantify amplicons but it is a complex and expensive technique that should be performed by highly trained personnel. PCR has been used in a wide variety of disciplines such as in food sciences, organ transplant, odontology, oncology and lately, as the standard diagnostic technique for COVID-19. Even when qPCR is a reliable and robust technique, it is hardly accessible for developing countries for its complex labelling procedures and expensive instrumentation, for that, it is of big relevance to search for simpler and cheaper alternative technologies for the detection and analysis of DNA. In this work, we explore the feasibility of using multifrequency bioimpedance measurements to detect label-free PCR products as a proof of principle for the future development of a gene biosensor on the basis of PCR and bioimpedance measurements.
AB - PCR is a molecular technique that multiplies DNA fragments in a logarithmical way. qPCR uses fluoroscopic dyes or probes to quantify amplicons but it is a complex and expensive technique that should be performed by highly trained personnel. PCR has been used in a wide variety of disciplines such as in food sciences, organ transplant, odontology, oncology and lately, as the standard diagnostic technique for COVID-19. Even when qPCR is a reliable and robust technique, it is hardly accessible for developing countries for its complex labelling procedures and expensive instrumentation, for that, it is of big relevance to search for simpler and cheaper alternative technologies for the detection and analysis of DNA. In this work, we explore the feasibility of using multifrequency bioimpedance measurements to detect label-free PCR products as a proof of principle for the future development of a gene biosensor on the basis of PCR and bioimpedance measurements.
UR - http://www.scopus.com/inward/record.url?scp=85120803306&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/2008/1/012016
DO - 10.1088/1742-6596/2008/1/012016
M3 - Artículo de la conferencia
AN - SCOPUS:85120803306
SN - 1742-6588
VL - 2008
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012016
T2 - 4th Latin American Conference on Bioimpedance 2021, CLABIO 2021
Y2 - 10 November 2021 through 13 November 2021
ER -