TY - JOUR
T1 - Biodiesel Production Catalyzed by Lipase Extract Powder of Leonotis nepetifolia (Christmas Candlestick) Seed
AU - Ávila Vázquez, Verónica
AU - Aguilera Flores, Miguel Mauricio
AU - Hernández Casas, Luis Felipe
AU - Medellín Castillo, Nahum Andrés
AU - Rocha Uribe, Alejandro
AU - Correa Aguado, Hans Christian
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/3
Y1 - 2023/3
N2 - This work aimed to evaluate the ability of lipase extract powder obtained from Leonotis nepetifolia seed for enzyme-catalyzed biodiesel production using Leonotis nepetifolia oil, commercial olive oil, and waste cooking oil as substrates. The lipase extract powder showed an enzymatic activity and hydrolysis percentage of 24.7 U/g and 21.31%, respectively, using commercial olive oil as a reference. Transesterification reaction conditions were 40 g of substrate, 34 °C, molar ratio oil: methanol of 1:3, lipase extract powder 20 wt%, phosphates buffer (pH 4.8) 20 wt%, and a reaction time of 8 h. Transesterification yields of 74.5%, 71.5%, and 69.3% for commercial olive oil, waste cooking oil, and Leonotis nepetifolia oil were obtained, respectively. Biodiesel physicochemical parameters were analyzed and compared with the international standards: EN 14214 (European Union) and ASTM D6751 (American Society for Testing and Materials). The biodiesel’s moisture and volatile matter percentages, iodine index, cooper strip corrosion, and methyl esters content conformed to the standards’ specifications. The fatty acid methyl ester content of the vegetable oils showed the presence of methyl oleate after enzyme-catalyzed transesterification. This study reveals that biodiesel production catalyzed by lipase extract powder from Leonotis nepetifolia could be a viable alternative, showing that transesterification yields competitive results.
AB - This work aimed to evaluate the ability of lipase extract powder obtained from Leonotis nepetifolia seed for enzyme-catalyzed biodiesel production using Leonotis nepetifolia oil, commercial olive oil, and waste cooking oil as substrates. The lipase extract powder showed an enzymatic activity and hydrolysis percentage of 24.7 U/g and 21.31%, respectively, using commercial olive oil as a reference. Transesterification reaction conditions were 40 g of substrate, 34 °C, molar ratio oil: methanol of 1:3, lipase extract powder 20 wt%, phosphates buffer (pH 4.8) 20 wt%, and a reaction time of 8 h. Transesterification yields of 74.5%, 71.5%, and 69.3% for commercial olive oil, waste cooking oil, and Leonotis nepetifolia oil were obtained, respectively. Biodiesel physicochemical parameters were analyzed and compared with the international standards: EN 14214 (European Union) and ASTM D6751 (American Society for Testing and Materials). The biodiesel’s moisture and volatile matter percentages, iodine index, cooper strip corrosion, and methyl esters content conformed to the standards’ specifications. The fatty acid methyl ester content of the vegetable oils showed the presence of methyl oleate after enzyme-catalyzed transesterification. This study reveals that biodiesel production catalyzed by lipase extract powder from Leonotis nepetifolia could be a viable alternative, showing that transesterification yields competitive results.
KW - biodiesel quality
KW - enzymatic activity
KW - enzyme-catalyzed transesterification
KW - hydrolysis percentage
UR - http://www.scopus.com/inward/record.url?scp=85151920777&partnerID=8YFLogxK
U2 - 10.3390/en16062848
DO - 10.3390/en16062848
M3 - Artículo
AN - SCOPUS:85151920777
SN - 1996-1073
VL - 16
JO - Energies
JF - Energies
IS - 6
M1 - 2848
ER -