TY - JOUR
T1 - Biodegradation of 2,4-dichlorophenol by a Bacillus consortium
AU - Herrera, Yesenia
AU - Okoh, Anthony I.
AU - Alvarez, Laura
AU - Robledo, Norma
AU - Trejo-Hernández, María R.
N1 - Funding Information:
Acknowledgements The authors would like to thank CONACYT for financial support (Project 26080, and Master’s studies fellowship 113390). Our appreciation also goes to S.P. Verma, J.A. Rocha for his constructive and insightful suggestions during the preparation of this manuscript.
PY - 2008/1
Y1 - 2008/1
N2 - As part of our effort at establishing microbial consortia of relevance for the bioremediation of xenobiotics polluted environments in Mexico, we assessed the aerobic biodegradation of 2,4-dichlorophenol (2,4-DCP) by a consortium of four Bacillus species that were isolated from a polluted soil by enrichment using a mixture of chlorophenols. The bacterial consortium effectively biodegraded 2-chlorophenol, 3-chlorophenol and 2,4-dichlorophenol at degradation rates of between 1.7 and 6.7 μmoles l-1 h-1. In the presence of NH4Cl or KNO2 as nitrogen sources, 2,4-DCP was variously degraded. Under both conditions, cell biomass attained highest values of 350 and 450 mg l-1 respectively, while the amounts of 2,4-DCP metabolized in 21 days reached peak values of 2.1 and 2.5 mM representing between 70 and 85% degradation respectively. Chloride releases during the same period were highest at 4.7 mM and 5.3 mM in the presence of the two nitrogen sources. The presence of free-chloride in the culture medium had a significant impact on the catabolism of 2,4-dichlorophenol.
AB - As part of our effort at establishing microbial consortia of relevance for the bioremediation of xenobiotics polluted environments in Mexico, we assessed the aerobic biodegradation of 2,4-dichlorophenol (2,4-DCP) by a consortium of four Bacillus species that were isolated from a polluted soil by enrichment using a mixture of chlorophenols. The bacterial consortium effectively biodegraded 2-chlorophenol, 3-chlorophenol and 2,4-dichlorophenol at degradation rates of between 1.7 and 6.7 μmoles l-1 h-1. In the presence of NH4Cl or KNO2 as nitrogen sources, 2,4-DCP was variously degraded. Under both conditions, cell biomass attained highest values of 350 and 450 mg l-1 respectively, while the amounts of 2,4-DCP metabolized in 21 days reached peak values of 2.1 and 2.5 mM representing between 70 and 85% degradation respectively. Chloride releases during the same period were highest at 4.7 mM and 5.3 mM in the presence of the two nitrogen sources. The presence of free-chloride in the culture medium had a significant impact on the catabolism of 2,4-dichlorophenol.
KW - 2,4-Dichlorophenol
KW - Bacillus consortium
KW - Biodegradation
KW - Nitrogen source
UR - http://www.scopus.com/inward/record.url?scp=38649094883&partnerID=8YFLogxK
U2 - 10.1007/s11274-007-9437-0
DO - 10.1007/s11274-007-9437-0
M3 - Artículo
SN - 0959-3993
VL - 24
SP - 55
EP - 60
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 1
ER -