TY - JOUR
T1 - Biocontrol potential and polyphasic characterization of novel native Trichoderma strains against Macrophomina phaseolina isolated from sorghum and common bean
AU - Larralde-Corona, C. P.
AU - Santiago-Mena, M. R.
AU - Sifuentes-Rincón, A. M.
AU - Rodríguez-Luna, I. C.
AU - Rodríguez-Pérez, M. A.
AU - Shirai, K.
AU - Narváez-Zapata, J. A.
N1 - Funding Information:
Acknowledgments We would like to acknowledge the technical support provided by A. Oliva-Hernández in ITS1–5.8S–ITS2 sequencing and the financial support of project funds CGPI2003-0180 and CGPI2005-0342 granted by the Instituto Politécnico Nacional (IPN, México).
PY - 2008/8
Y1 - 2008/8
N2 - Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of β-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.
AB - Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of β-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.
KW - Biocontrol
KW - Macrophomina phaseolina
KW - Polyphasic characterization
KW - Trichoderma isolates
UR - http://www.scopus.com/inward/record.url?scp=47149094625&partnerID=8YFLogxK
U2 - 10.1007/s00253-008-1532-0
DO - 10.1007/s00253-008-1532-0
M3 - Artículo
C2 - 18523764
SN - 0175-7598
VL - 80
SP - 167
EP - 177
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1
ER -