TY - JOUR
T1 - Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03
AU - Morales-Ruiz, Estefaniá
AU - Priego-Rivera, Ricardo
AU - Figueroa-López, Alejandro Miguel
AU - Cazares-Álvarez, Jesús Eduardo
AU - Maldonado-Mendoza, Ignacio E.
N1 - Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides.
AB - Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides.
KW - biocontrol
KW - chitinase
KW - maize
KW - phytopathogen
KW - rhizosphere
UR - http://www.scopus.com/inward/record.url?scp=85102018766&partnerID=8YFLogxK
U2 - 10.1093/femsle/fnaa218
DO - 10.1093/femsle/fnaa218
M3 - Artículo
C2 - 33351136
AN - SCOPUS:85102018766
SN - 0378-1097
VL - 368
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
M1 - fnaa218
ER -