TY - JOUR
T1 - Beneficial effect of retinoic acid on the outcome of experimental acute renal failure
AU - Perez, Alejandro
AU - Ramirez-Ramos, Marisela
AU - Calleja, Consuelo
AU - Martin, Dolores
AU - Namorado, Maria C.
AU - Sierra, Gerardo
AU - Ramirez-Ramos, María E.
AU - Paniagua, Ramon
AU - Sánchez, Yadira
AU - Arreola, Laura
AU - Reyes, Jose L.
N1 - Funding Information:
Acknowledgements. The authors gratefully acknowledge the skilful secretarial assistance of Elvia Hernandez. This work was partially supported by a grant from Consejo Nacional de Ciencia y Tecnología (G34511M).
PY - 2004/10
Y1 - 2004/10
N2 - Background. Retinoic acid (RA) exerts beneficial effects on vascular remodelling and experimental nephritis, and plays a role in kidney development. Pathological changes caused by acute renal failure (ARF) result in high mortality. We determined whether RA ameliorates ARF-induced pathology caused by potassium dichromate (PD). Methods. Adult Wistar female rats (210-250g) were randomly allocated to four groups: (i) an ARF group that received PD [15 mg/kg body weight (bw), single dose subcutaneously]; (ii) a group that received PD plus RA (1 mg/kg bw) beginning at 5 days before PD and that continued for 14 additional days; (iii) a group that received PD plus thyroxine (T4; 8 μg/100 g bw) with RA; and (iv) a group that received only the vehicle for PD (saline solution). We evaluated functional, biochemical and morphological characteristics of the kidneys. Results. PD-induced alterations in serum creatinine, creatinine clearance (Ccr) and fractional excretion of sodium (FeNa) were less severe when rats received RA. PD increased lipoperoxidation and this alteration was partially blocked by RA. Animals undergoing ARF showed severe histological injury (brush border loss, acidophilia, oedema, pyknosis, karyorhexis, cell detachment and disruption of the basement membrane). These alterations were less severe in RA-treated rats, indicating a protective effect on functional and morphological alterations. Alterations in urinary sediment were reduced by RA. The simultaneous administration of T4 with RA did not produce additional protection. Conclusion. RA exerted beneficial effects on the duration and severity of renal damage induced by PD in a model of renal failure resembling ARF in humans. The protective effect of RA may be mediated by diminished lipoperoxidative damage.
AB - Background. Retinoic acid (RA) exerts beneficial effects on vascular remodelling and experimental nephritis, and plays a role in kidney development. Pathological changes caused by acute renal failure (ARF) result in high mortality. We determined whether RA ameliorates ARF-induced pathology caused by potassium dichromate (PD). Methods. Adult Wistar female rats (210-250g) were randomly allocated to four groups: (i) an ARF group that received PD [15 mg/kg body weight (bw), single dose subcutaneously]; (ii) a group that received PD plus RA (1 mg/kg bw) beginning at 5 days before PD and that continued for 14 additional days; (iii) a group that received PD plus thyroxine (T4; 8 μg/100 g bw) with RA; and (iv) a group that received only the vehicle for PD (saline solution). We evaluated functional, biochemical and morphological characteristics of the kidneys. Results. PD-induced alterations in serum creatinine, creatinine clearance (Ccr) and fractional excretion of sodium (FeNa) were less severe when rats received RA. PD increased lipoperoxidation and this alteration was partially blocked by RA. Animals undergoing ARF showed severe histological injury (brush border loss, acidophilia, oedema, pyknosis, karyorhexis, cell detachment and disruption of the basement membrane). These alterations were less severe in RA-treated rats, indicating a protective effect on functional and morphological alterations. Alterations in urinary sediment were reduced by RA. The simultaneous administration of T4 with RA did not produce additional protection. Conclusion. RA exerted beneficial effects on the duration and severity of renal damage induced by PD in a model of renal failure resembling ARF in humans. The protective effect of RA may be mediated by diminished lipoperoxidative damage.
KW - Acute renal failure
KW - Creatinine clearance
KW - FeNa
KW - Potassium dichronate
KW - Retinoic acid
KW - T
UR - http://www.scopus.com/inward/record.url?scp=5444247799&partnerID=8YFLogxK
U2 - 10.1093/ndt/gfh400
DO - 10.1093/ndt/gfh400
M3 - Artículo
SN - 0931-0509
VL - 19
SP - 2464
EP - 2471
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 10
ER -