TY - JOUR
T1 - Assessment of non-cultured aquatic fungal diversity from different habitats in Mexico
AU - Valderrama, Brenda
AU - Paredes-Valdez, Guadalupe
AU - Rodríguez, Rocío
AU - Romero-Guido, Cynthia
AU - Martínez, Fernando
AU - Martínez-Romero, Julio
AU - Guerrero-Galván, Saúl
AU - Mendoza-Herrera, Alberto
AU - Folch-Mallol, Jorge Luis
N1 - Publisher Copyright:
© 2016 Universidad Nacional Autónoma de México, Instituto de Biología.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - With the aim to explore the diversity of aquatic fungi in Mexico we present an investigation using a fragment of the 18S ribosomal DNA as a molecular marker obtained from different water bodies (marine, brackish and fresh water). Ribosomal gene fragments were obtained by DNA amplification, the resulting sequences were compared using multiple alignments against a collection of classified reference fungal sequences and then subjected to phylogenetic clustering allowing the identification and classification of DNA sequences from environmental isolates as fungal down to the family level, provided enough reference sequence were available. From our ensemble of 2,020 sequences identified as fungal, 23.8% were classified at the family level, 48.5% at the order level, 13% at the class/subphylum level and 14.7% of the sequences (all from the same site) could not be unambiguously positioned in any of our reference fungal groups but were closely related to uncultivated marine fungi. The most frequently recovered phylum was Ascomycota (89.1%), followed by Chytridiomycota (8.1%), Basidiomycota (2.8%) and Mucoromycotina (1.3%).
AB - With the aim to explore the diversity of aquatic fungi in Mexico we present an investigation using a fragment of the 18S ribosomal DNA as a molecular marker obtained from different water bodies (marine, brackish and fresh water). Ribosomal gene fragments were obtained by DNA amplification, the resulting sequences were compared using multiple alignments against a collection of classified reference fungal sequences and then subjected to phylogenetic clustering allowing the identification and classification of DNA sequences from environmental isolates as fungal down to the family level, provided enough reference sequence were available. From our ensemble of 2,020 sequences identified as fungal, 23.8% were classified at the family level, 48.5% at the order level, 13% at the class/subphylum level and 14.7% of the sequences (all from the same site) could not be unambiguously positioned in any of our reference fungal groups but were closely related to uncultivated marine fungi. The most frequently recovered phylum was Ascomycota (89.1%), followed by Chytridiomycota (8.1%), Basidiomycota (2.8%) and Mucoromycotina (1.3%).
KW - 18S ribosomal DNA
KW - Aquatic habitats
KW - Fungal populations
KW - Fungi
KW - Taxonomic classification
UR - http://www.scopus.com/inward/record.url?scp=85032094694&partnerID=8YFLogxK
U2 - 10.1016/j.rmb.2016.01.013
DO - 10.1016/j.rmb.2016.01.013
M3 - Artículo
SN - 1870-3453
VL - 87
SP - 18
EP - 28
JO - Revista Mexicana de Biodiversidad
JF - Revista Mexicana de Biodiversidad
IS - 1
ER -