TY - JOUR
T1 - Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples
AU - Fuentes-Pananá, Ezequiel M.
AU - Larios-Serrato, Violeta
AU - Méndez-Tenorio, Alfonso
AU - Morales-Sánchez, Abigail
AU - Arias, Carlos F.
AU - Torres, Javier
N1 - Publisher Copyright:
© 2016, Fundacao Oswaldo Cruz. All rights reserved.
PY - 2016/3
Y1 - 2016/3
N2 - Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus-cancer associations as well as to discovery new tumour viruses. The objective of this study was to document possible associations of viral infection with GC and BrC in Mexican patients. In order to gain idea about cost effective conditions of experimental sequencing, we first carried out an in silico simulation of WGS. The next-generation-platform IlluminaGallx was then used to sequence GC and BrC tumour samples. While we did not find viral sequences in tissues from BrC patients, multiple reads matching Epstein-Barr virus (EBV) sequences were found in GC tissues. An end-point polymerase chain reaction confirmed an enrichment of EBV sequences in one of the GC samples sequenced, validating the next-generation sequencing-bioinformatics pipeline.
AB - Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus-cancer associations as well as to discovery new tumour viruses. The objective of this study was to document possible associations of viral infection with GC and BrC in Mexican patients. In order to gain idea about cost effective conditions of experimental sequencing, we first carried out an in silico simulation of WGS. The next-generation-platform IlluminaGallx was then used to sequence GC and BrC tumour samples. While we did not find viral sequences in tissues from BrC patients, multiple reads matching Epstein-Barr virus (EBV) sequences were found in GC tissues. An end-point polymerase chain reaction confirmed an enrichment of EBV sequences in one of the GC samples sequenced, validating the next-generation sequencing-bioinformatics pipeline.
KW - Breast cancer
KW - EBV
KW - Gastric cancer
KW - Gastritis
KW - Wide genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=84960338824&partnerID=8YFLogxK
U2 - 10.1590/0074-02760150405
DO - 10.1590/0074-02760150405
M3 - Artículo
C2 - 26910355
SN - 0074-0276
VL - 111
SP - 200
EP - 208
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
IS - 3
ER -