TY - JOUR
T1 - Asociación molecular y función del agente tensioactivo pulmonar de ternera
AU - Montaño, María Del Lurdez Consuelo Martínez
AU - Sánchez, José Luis Muñoz
AU - Ramírez, Isabel Baeza
PY - 2007/4
Y1 - 2007/4
N2 - Surfactant, a highly surface-active material composed of phospholipids, neutral lipids and proteins, lines the lungs' alveolar surface facilitating alveolar ventilation. The molecular organization of surfactant components isolated from calf-lungs was analyzed by differential-scanning calorimetry and dynamic light-scattering, and subsequently compared to surfactant components organized in uni and multilamellar liposomes. The respiratory distress syndrome developed in adult guinea pigs was used for assessing surfactant activity. Calorimetry studies showed that lipidprotein interactions were considerably abated in native surfactant as compared to those of surfactant in uni or multi-lamellar liposomes. Light-scattering experiments indicated that native surfactant has a fibrillar shape with limited lipid-protein interactions, suggesting that it is organized in a lattice-like structure forming a stable film. These findings underscore the importance of the native molecular organization of surfactant. When surfactant reconstituted as uni- or multilamellar liposomes was administred to animals under respiratory distress, they did not recover. In contrast, when native surfactant was used to treat sick animals, arterial pH and PaCO2 values improved, almost reaching normal values. It is important to emphasize that fewer steps in the protocol for isolation of calf lung surfactant made it possible to obtain it in a physiologically active molecular form.
AB - Surfactant, a highly surface-active material composed of phospholipids, neutral lipids and proteins, lines the lungs' alveolar surface facilitating alveolar ventilation. The molecular organization of surfactant components isolated from calf-lungs was analyzed by differential-scanning calorimetry and dynamic light-scattering, and subsequently compared to surfactant components organized in uni and multilamellar liposomes. The respiratory distress syndrome developed in adult guinea pigs was used for assessing surfactant activity. Calorimetry studies showed that lipidprotein interactions were considerably abated in native surfactant as compared to those of surfactant in uni or multi-lamellar liposomes. Light-scattering experiments indicated that native surfactant has a fibrillar shape with limited lipid-protein interactions, suggesting that it is organized in a lattice-like structure forming a stable film. These findings underscore the importance of the native molecular organization of surfactant. When surfactant reconstituted as uni- or multilamellar liposomes was administred to animals under respiratory distress, they did not recover. In contrast, when native surfactant was used to treat sick animals, arterial pH and PaCO2 values improved, almost reaching normal values. It is important to emphasize that fewer steps in the protocol for isolation of calf lung surfactant made it possible to obtain it in a physiologically active molecular form.
KW - Calf lung surfactant
KW - Differential scanning calorimetry
KW - Light scattering
KW - Multilamellar liposomes
KW - Respiratory distress syndrome
KW - Unilamellar liposomes
UR - http://www.scopus.com/inward/record.url?scp=36849091907&partnerID=8YFLogxK
M3 - Artículo
SN - 0325-2957
VL - 41
SP - 237
EP - 245
JO - Acta Bioquimica Clinica Latinoamericana
JF - Acta Bioquimica Clinica Latinoamericana
IS - 2
ER -