TY - JOUR
T1 - Arsenate induces the expression of fungal genes involved in As transport in arbuscular mycorrhiza
AU - González-Chávez, Ma del Carmen A.
AU - Ortega-Larrocea, María del Pilar
AU - Carrillo-González, Rogelio
AU - López-Meyer, Melina
AU - Xoconostle-Cázares, Beatriz
AU - Gomez, Susana K.
AU - Harrison, Maria J.
AU - Figueroa-López, Alejandro Miguel
AU - Maldonado-Mendoza, Ignacio E.
N1 - Funding Information:
We thank L. Gómez-Flores for her technical assistance. This project was supported by a competitive grant from the SEMARNAT-CONACyT Fund ( SEMARNAT-2002-C01-0095 ) and Collaborative Research Grant Program Texas A&M-CONACyT (2009-021). We also thank M.Sc. R. Cervantes Gámez and K.Y. Leyva-Madrigal for technical assistance with RT-PCR and phylogenetic analysis, respectively. Dr J.P. Bernal helped with the ICP determinations. AMFL had a fellowship from CONACyT for his undergraduate studies and is currently a PIFI scholar from IPN.
PY - 2011/12
Y1 - 2011/12
N2 - We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correlates with As(V) uptake in the extra-radical mycelium of G. intraradices. We showed a time-concerted induction of transcript levels first of GiPT, followed by GiArsA, as well as the location of gene expression using laser microdissection of these two genes not only in the extra-radical mycelium but also in arbuscules. This work represents the first report showing the dissection of the molecular players involved in arbuscular mycorrhizal fungus (AMF)-mediated As tolerance in plants, and suggests that tolerance mediated by AMF may be caused by an As exclusion mechanism, where fungal structures such as the extra-radical mycelium and arbuscules may be playing an important role. Our results extend knowledge of the mechanisms underlying As efflux in arbuscular mycorrhizal fungi and mechanisms related to As tolerance.
AB - We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correlates with As(V) uptake in the extra-radical mycelium of G. intraradices. We showed a time-concerted induction of transcript levels first of GiPT, followed by GiArsA, as well as the location of gene expression using laser microdissection of these two genes not only in the extra-radical mycelium but also in arbuscules. This work represents the first report showing the dissection of the molecular players involved in arbuscular mycorrhizal fungus (AMF)-mediated As tolerance in plants, and suggests that tolerance mediated by AMF may be caused by an As exclusion mechanism, where fungal structures such as the extra-radical mycelium and arbuscules may be playing an important role. Our results extend knowledge of the mechanisms underlying As efflux in arbuscular mycorrhizal fungi and mechanisms related to As tolerance.
KW - Arbuscular mycorrhizal fungus tolerance to arsenic
KW - Arsenate exclusion
KW - Giarsa gene
KW - High-affinity pi/as transporter
UR - http://www.scopus.com/inward/record.url?scp=81955160706&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2011.08.005
DO - 10.1016/j.funbio.2011.08.005
M3 - Artículo
C2 - 22115439
SN - 1878-6146
VL - 115
SP - 1197
EP - 1209
JO - Fungal Biology
JF - Fungal Biology
IS - 12
ER -