TY - JOUR
T1 - Antioxidant and antimutagenic activities of Randia echinocarpa fruit
AU - Santos-Cervantes, María Elena
AU - Ibarra-Zazueta, María Emilia
AU - Loarca-Piña, Guadalupe
AU - Paredes-López, Octavio
AU - Delgado-Vargas, Francisco
N1 - Funding Information:
Acknowledgements We acknowledge the help of the following institutions/ persons: Consejo Nacional de Ciencia y Tecnología, CECYT, SEP and UAS for the financial support; Dr. Rito Vega-Aviña, Agronomy Faculty, UAS, Culiacan, Sinaloa, Mexico, for his help in the taxonomical identification of the plant material, and Jose A. Lopez-Valenzuela, Faculty of Chemical and Biological Sciences, UAS, Culiacan, Sinaloa, Mexico, for his technical assistance.
PY - 2007/6
Y1 - 2007/6
N2 - We report for the first time the antioxidant and antimutagenic activities of fractions from Randia echinocarpa fruit, which is a Rubiaceae plant native to Sinaloa, Mexico. This fruit has been traditionally used in the prevention or treatment of cancer, among other diseases. The pulp of the fruit was sequentially extracted with solvents of different polarity (i.e. hexane, chloroform, methanol and water). A high extraction yield was obtained with methanol (72.17% d.w.). The aqueous extract showed the highest content of phenolics (2.27 mg/g as ferulic acid equivalents) and the highest antioxidant activity based on the β-carotene bleaching method (486.15). The commercial antioxidant BHT was used as control (835.05). Antimutagenic activity of the aqueous extract (0-500 μg/tube) was evaluated using the Salmonella microsuspension assay (YG1024 strain) and 1-NP as the mutagen (50 and 100 ng/tube). The aqueous extract was neither toxic nor mutagenic and the percentage of inhibition on 1-NP mutagenicity was 32 and 53% at doses of 50 and 100 ng/tube, respectively. The results of the double incubation assay suggest that the extract inhibited the mutagenicity of 1-NP by a combination of desmutagenic and bioantimutagenic effects.
AB - We report for the first time the antioxidant and antimutagenic activities of fractions from Randia echinocarpa fruit, which is a Rubiaceae plant native to Sinaloa, Mexico. This fruit has been traditionally used in the prevention or treatment of cancer, among other diseases. The pulp of the fruit was sequentially extracted with solvents of different polarity (i.e. hexane, chloroform, methanol and water). A high extraction yield was obtained with methanol (72.17% d.w.). The aqueous extract showed the highest content of phenolics (2.27 mg/g as ferulic acid equivalents) and the highest antioxidant activity based on the β-carotene bleaching method (486.15). The commercial antioxidant BHT was used as control (835.05). Antimutagenic activity of the aqueous extract (0-500 μg/tube) was evaluated using the Salmonella microsuspension assay (YG1024 strain) and 1-NP as the mutagen (50 and 100 ng/tube). The aqueous extract was neither toxic nor mutagenic and the percentage of inhibition on 1-NP mutagenicity was 32 and 53% at doses of 50 and 100 ng/tube, respectively. The results of the double incubation assay suggest that the extract inhibited the mutagenicity of 1-NP by a combination of desmutagenic and bioantimutagenic effects.
KW - Antimutagenic activity
KW - Antioxidant activity
KW - Desmutagen and bioantimutagen
KW - Randia echinocarpa
KW - Salmonella typhimurium
KW - Total phenolics
UR - http://www.scopus.com/inward/record.url?scp=34447544495&partnerID=8YFLogxK
U2 - 10.1007/s11130-007-0044-x
DO - 10.1007/s11130-007-0044-x
M3 - Artículo
C2 - 17577670
AN - SCOPUS:34447544495
SN - 0921-9668
VL - 62
SP - 71
EP - 77
JO - Plant Foods for Human Nutrition
JF - Plant Foods for Human Nutrition
IS - 2
ER -