Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity

M. De Lourdes Munoz, E. Lamoyi, G. Leon, R. Tovar, J. Perez-Garcia, M. De La Torre, E. Murueta, R. M. Bernal

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In vitro interaction of Entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (EDG) and stimulation of collagenolytic activity. Purified EDG contain 1.66 U of collagenase per mg of protein. Thus, EDG may participate in tissue destruction during invasive amebiasis. Monoclonal antibodies (MAbs) L1.1 and L7.1 reacted specifically with EDG in enzyme-linked immunosorbent assay (ELISA) and immunofluorescence and immunoelectron microscopy. MAb L7.1 immunoprecipitated three polypeptides with molecular weights of 95,000, 68,000, and 28,000 from lysates of biosynthetically labeled E. histolytica. Both MAbs recognized the pathogenic E. histolytica axenic strains HM1:IMSS, HM 38:IMSS, and HK-9 but failed to react in ELISA with Entamoeba moshkovskii, Entamoeba invadens, and E. histolytica-like Laredo. In addition, MAb L7.1 reacted with one E. histolytica isolate from a symptomatic patient bud did not react with four of five isolates from asymptomatic patients. EDG antigens were detected by a MAb L7.1-based ELISA in E. histolytica-containing fecal samples from symptomatic, but not asymptomatic, individuals. These results suggest that the EDG antigen detected with MAb L7.1 may be differentially expressed in pathogenic and nonpathogenic E. histolytica.
Original languageAmerican English
Pages (from-to)2418-2424
Number of pages2175
JournalJournal of Clinical Microbiology
StatePublished - 5 Nov 1990

Fingerprint

Entamoeba histolytica
antigens
Antigens
markers
Virulence
Electrons
Immunosorbents
enzymes
Assays
electrons
Entamoeba
Monoclonal antibodies
Enzymes
antibodies
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Polypeptides
polypeptides
Amebiasis
Collagenases

Cite this

De Lourdes Munoz, M., Lamoyi, E., Leon, G., Tovar, R., Perez-Garcia, J., De La Torre, M., ... Bernal, R. M. (1990). Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity. Journal of Clinical Microbiology, 2418-2424.
De Lourdes Munoz, M. ; Lamoyi, E. ; Leon, G. ; Tovar, R. ; Perez-Garcia, J. ; De La Torre, M. ; Murueta, E. ; Bernal, R. M. / Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity. In: Journal of Clinical Microbiology. 1990 ; pp. 2418-2424.
@article{4047be583d184c6cbec674168abb0a89,
title = "Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity",
abstract = "In vitro interaction of Entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (EDG) and stimulation of collagenolytic activity. Purified EDG contain 1.66 U of collagenase per mg of protein. Thus, EDG may participate in tissue destruction during invasive amebiasis. Monoclonal antibodies (MAbs) L1.1 and L7.1 reacted specifically with EDG in enzyme-linked immunosorbent assay (ELISA) and immunofluorescence and immunoelectron microscopy. MAb L7.1 immunoprecipitated three polypeptides with molecular weights of 95,000, 68,000, and 28,000 from lysates of biosynthetically labeled E. histolytica. Both MAbs recognized the pathogenic E. histolytica axenic strains HM1:IMSS, HM 38:IMSS, and HK-9 but failed to react in ELISA with Entamoeba moshkovskii, Entamoeba invadens, and E. histolytica-like Laredo. In addition, MAb L7.1 reacted with one E. histolytica isolate from a symptomatic patient bud did not react with four of five isolates from asymptomatic patients. EDG antigens were detected by a MAb L7.1-based ELISA in E. histolytica-containing fecal samples from symptomatic, but not asymptomatic, individuals. These results suggest that the EDG antigen detected with MAb L7.1 may be differentially expressed in pathogenic and nonpathogenic E. histolytica.",
author = "{De Lourdes Munoz}, M. and E. Lamoyi and G. Leon and R. Tovar and J. Perez-Garcia and {De La Torre}, M. and E. Murueta and Bernal, {R. M.}",
year = "1990",
month = "11",
day = "5",
language = "American English",
pages = "2418--2424",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",

}

De Lourdes Munoz, M, Lamoyi, E, Leon, G, Tovar, R, Perez-Garcia, J, De La Torre, M, Murueta, E & Bernal, RM 1990, 'Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity', Journal of Clinical Microbiology, pp. 2418-2424.

Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity. / De Lourdes Munoz, M.; Lamoyi, E.; Leon, G.; Tovar, R.; Perez-Garcia, J.; De La Torre, M.; Murueta, E.; Bernal, R. M.

In: Journal of Clinical Microbiology, 05.11.1990, p. 2418-2424.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity

AU - De Lourdes Munoz, M.

AU - Lamoyi, E.

AU - Leon, G.

AU - Tovar, R.

AU - Perez-Garcia, J.

AU - De La Torre, M.

AU - Murueta, E.

AU - Bernal, R. M.

PY - 1990/11/5

Y1 - 1990/11/5

N2 - In vitro interaction of Entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (EDG) and stimulation of collagenolytic activity. Purified EDG contain 1.66 U of collagenase per mg of protein. Thus, EDG may participate in tissue destruction during invasive amebiasis. Monoclonal antibodies (MAbs) L1.1 and L7.1 reacted specifically with EDG in enzyme-linked immunosorbent assay (ELISA) and immunofluorescence and immunoelectron microscopy. MAb L7.1 immunoprecipitated three polypeptides with molecular weights of 95,000, 68,000, and 28,000 from lysates of biosynthetically labeled E. histolytica. Both MAbs recognized the pathogenic E. histolytica axenic strains HM1:IMSS, HM 38:IMSS, and HK-9 but failed to react in ELISA with Entamoeba moshkovskii, Entamoeba invadens, and E. histolytica-like Laredo. In addition, MAb L7.1 reacted with one E. histolytica isolate from a symptomatic patient bud did not react with four of five isolates from asymptomatic patients. EDG antigens were detected by a MAb L7.1-based ELISA in E. histolytica-containing fecal samples from symptomatic, but not asymptomatic, individuals. These results suggest that the EDG antigen detected with MAb L7.1 may be differentially expressed in pathogenic and nonpathogenic E. histolytica.

AB - In vitro interaction of Entamoeba histolytica with collagen induces intracellular formation and release of electron-dense granules (EDG) and stimulation of collagenolytic activity. Purified EDG contain 1.66 U of collagenase per mg of protein. Thus, EDG may participate in tissue destruction during invasive amebiasis. Monoclonal antibodies (MAbs) L1.1 and L7.1 reacted specifically with EDG in enzyme-linked immunosorbent assay (ELISA) and immunofluorescence and immunoelectron microscopy. MAb L7.1 immunoprecipitated three polypeptides with molecular weights of 95,000, 68,000, and 28,000 from lysates of biosynthetically labeled E. histolytica. Both MAbs recognized the pathogenic E. histolytica axenic strains HM1:IMSS, HM 38:IMSS, and HK-9 but failed to react in ELISA with Entamoeba moshkovskii, Entamoeba invadens, and E. histolytica-like Laredo. In addition, MAb L7.1 reacted with one E. histolytica isolate from a symptomatic patient bud did not react with four of five isolates from asymptomatic patients. EDG antigens were detected by a MAb L7.1-based ELISA in E. histolytica-containing fecal samples from symptomatic, but not asymptomatic, individuals. These results suggest that the EDG antigen detected with MAb L7.1 may be differentially expressed in pathogenic and nonpathogenic E. histolytica.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025155833&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0025155833&origin=inward

M3 - Article

SP - 2418

EP - 2424

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

ER -

De Lourdes Munoz M, Lamoyi E, Leon G, Tovar R, Perez-Garcia J, De La Torre M et al. Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity. Journal of Clinical Microbiology. 1990 Nov 5;2418-2424.