Antigenotoxic effect of Chamomilla recutita (L.) rauschert essential oil in mouse spermatogonial cells, and determination of its antioxidant capacity in Vitro

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known as chamomile, is a plant used in traditional medicine for various therapeutic purposes. Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damage produced by mutagens in mice somatic cells. The aim of this research was to determine the inhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) in mice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on the rate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of the mutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after the damage induced by DAU; from the lowest to the highest dose of CEO we found an inhibition of 47.5%, 61.9%, and 93.5%, respectively. As a possible mechanism of action, the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test we observed a moderate scavenging potential of the oil; nevertheless, the second assay showed an antioxidant capacity similar to that observed with vitamin E. In conclusion, we found that CEO is an efficient chemoprotective agent against the damage induced by DAU in the precursor cells of the germinal line of mice, and that its antioxidant capacity may induce this effect.© 2010 by the authors; licensee MDPI, Basel.
Original languageAmerican English
Pages (from-to)3793-3802
Number of pages10
JournalInternational Journal of Molecular Sciences
DOIs
StatePublished - 1 Oct 2010

Fingerprint

Matricaria
Chamomile
Essential oils
antioxidants
Volatile Oils
Antioxidants
mice
oils
Daunorubicin
cells
Mutagens
mutagens
Sister Chromatid Exchange
damage
Scavenging
scavenging
Assays
Spermatogonia
Asteraceae
tocopherol

Cite this

@article{328e4ede358f411eab194f9c5b0683d1,
title = "Antigenotoxic effect of Chamomilla recutita (L.) rauschert essential oil in mouse spermatogonial cells, and determination of its antioxidant capacity in Vitro",
abstract = "Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known as chamomile, is a plant used in traditional medicine for various therapeutic purposes. Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damage produced by mutagens in mice somatic cells. The aim of this research was to determine the inhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) in mice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on the rate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of the mutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after the damage induced by DAU; from the lowest to the highest dose of CEO we found an inhibition of 47.5{\%}, 61.9{\%}, and 93.5{\%}, respectively. As a possible mechanism of action, the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test we observed a moderate scavenging potential of the oil; nevertheless, the second assay showed an antioxidant capacity similar to that observed with vitamin E. In conclusion, we found that CEO is an efficient chemoprotective agent against the damage induced by DAU in the precursor cells of the germinal line of mice, and that its antioxidant capacity may induce this effect.{\circledC} 2010 by the authors; licensee MDPI, Basel.",
author = "Alejandra Hern{\'a}ndez-Ceruelos and Eduardo Madrigal-Santill{\'a}n and Morales-Gonz{\'a}lez, {Jos{\'e} Antonio} and Germ{\'a}n Chamorro-Cevallos and Martha Cassani-Galindo and Eduardo Madrigal-Bujaidar",
year = "2010",
month = "10",
day = "1",
doi = "10.3390/ijms11103793",
language = "American English",
pages = "3793--3802",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

}

TY - JOUR

T1 - Antigenotoxic effect of Chamomilla recutita (L.) rauschert essential oil in mouse spermatogonial cells, and determination of its antioxidant capacity in Vitro

AU - Hernández-Ceruelos, Alejandra

AU - Madrigal-Santillán, Eduardo

AU - Morales-González, José Antonio

AU - Chamorro-Cevallos, Germán

AU - Cassani-Galindo, Martha

AU - Madrigal-Bujaidar, Eduardo

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known as chamomile, is a plant used in traditional medicine for various therapeutic purposes. Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damage produced by mutagens in mice somatic cells. The aim of this research was to determine the inhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) in mice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on the rate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of the mutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after the damage induced by DAU; from the lowest to the highest dose of CEO we found an inhibition of 47.5%, 61.9%, and 93.5%, respectively. As a possible mechanism of action, the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test we observed a moderate scavenging potential of the oil; nevertheless, the second assay showed an antioxidant capacity similar to that observed with vitamin E. In conclusion, we found that CEO is an efficient chemoprotective agent against the damage induced by DAU in the precursor cells of the germinal line of mice, and that its antioxidant capacity may induce this effect.© 2010 by the authors; licensee MDPI, Basel.

AB - Chamomilla recutita (L.) Rauschert (Asteraceae), popularly known as chamomile, is a plant used in traditional medicine for various therapeutic purposes. Chamomile essential oil (CEO) is particularly known to inhibit the genotoxic damage produced by mutagens in mice somatic cells. The aim of this research was to determine the inhibitory potential of CEO on the genotoxic damage produced by daunorubicin (DAU) in mice germ cells. We evaluated the effect of 5, 50, and 500 mg/kg of essential oil on the rate of sister chromatid exchange (SCE) induced in spermatogonia by 10 mg/kg of the mutagen. We found no genotoxicity of CEO, but detected an inhibition of SCE after the damage induced by DAU; from the lowest to the highest dose of CEO we found an inhibition of 47.5%, 61.9%, and 93.5%, respectively. As a possible mechanism of action, the antioxidant capacity of CEO was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method and ferric thiocyanate assays. In the first test we observed a moderate scavenging potential of the oil; nevertheless, the second assay showed an antioxidant capacity similar to that observed with vitamin E. In conclusion, we found that CEO is an efficient chemoprotective agent against the damage induced by DAU in the precursor cells of the germinal line of mice, and that its antioxidant capacity may induce this effect.© 2010 by the authors; licensee MDPI, Basel.

U2 - 10.3390/ijms11103793

DO - 10.3390/ijms11103793

M3 - Article

SP - 3793

EP - 3802

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

ER -