TY - JOUR
T1 - Antibacterial activity of aristolochia brevipes against multidrug-resistant mycobacterium tuberculosis
AU - Navarro-García, Víctor Manuel
AU - Luna-Herrera, Julieta
AU - Rojas-Bribiesca, Ma Gabriela
AU - Álvarez-Fitz, Patricia
AU - Ríos, María Yolanda
PY - 2011/9
Y1 - 2011/9
N2 - The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6α-7-dehydro-N-formylnornantenine; (2) E/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxytariacuripyrone; (5) aristololactam I; (6) β-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H-and 13C-(1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 μg/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 μg/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.
AB - The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6α-7-dehydro-N-formylnornantenine; (2) E/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxytariacuripyrone; (5) aristololactam I; (6) β-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H-and 13C-(1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 μg/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 μg/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.
KW - Alamarblue™ microassay
KW - Aristolochia brevipes
KW - Medicinal plant
KW - Multidrug-resistant (MDR)
KW - Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=80053262089&partnerID=8YFLogxK
U2 - 10.3390/molecules16097357
DO - 10.3390/molecules16097357
M3 - Artículo
C2 - 21876482
SN - 1420-3049
VL - 16
SP - 7357
EP - 7364
JO - Molecules
JF - Molecules
IS - 9
ER -