TY - JOUR
T1 - Angiotensin-converting enzyme inhibitory and antioxidative activities and functional characterization of protein hydrolysates of hard-to-cook chickpeas
AU - Medina-Godoy, Sergio
AU - Ambriz-Pérez, Dulce L.
AU - Fuentes-Gutiérrez, Cindy I.
AU - Germán-Báez, Lourdes J.
AU - Gutiérrez-Dorado, Roberto
AU - Reyes-Moreno, Cuauhtémoc
AU - Valdez-Ortiz, Angel
PY - 2012/7
Y1 - 2012/7
N2 - Background: The potential use of hard-to-cook (hardened) chickpeas to obtain value-added functional food ingredients was evaluated. For that purpose, some nutraceutical and functional attributes of several chickpea protein hydrolysates (CPHs) prepared from both fresh and hard-to-cook grains were evaluated. Results: All the CPHs prepared from both fresh and hard-to-cook grains, with the enzymes alcalase, pancreatin and papain, showed high angiotensin converting enzyme inhibitory (ACE-I) activity with IC 50 values ranging from 0.101 to 37.33 μg mL -1; similarly, high levels of antioxidant activity (around 18.17-95.61 μmol Trolox equivalent antioxidant capacity μg -1 CPH) were obtained through both the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods. Regarding functional characterization of the CPHs, oil absorption values ranged from 1.91 to 2.20 mL oil g -1 CPH, with water solubility almost 100% from pH 7 to 10. Conclusion: The high antioxidant and ACE-I activities as well as the good functional properties of the CPH prepared from both fresh and hard-to-cook grains, suggest its use in food formulations with value added in human health.
AB - Background: The potential use of hard-to-cook (hardened) chickpeas to obtain value-added functional food ingredients was evaluated. For that purpose, some nutraceutical and functional attributes of several chickpea protein hydrolysates (CPHs) prepared from both fresh and hard-to-cook grains were evaluated. Results: All the CPHs prepared from both fresh and hard-to-cook grains, with the enzymes alcalase, pancreatin and papain, showed high angiotensin converting enzyme inhibitory (ACE-I) activity with IC 50 values ranging from 0.101 to 37.33 μg mL -1; similarly, high levels of antioxidant activity (around 18.17-95.61 μmol Trolox equivalent antioxidant capacity μg -1 CPH) were obtained through both the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods. Regarding functional characterization of the CPHs, oil absorption values ranged from 1.91 to 2.20 mL oil g -1 CPH, with water solubility almost 100% from pH 7 to 10. Conclusion: The high antioxidant and ACE-I activities as well as the good functional properties of the CPH prepared from both fresh and hard-to-cook grains, suggest its use in food formulations with value added in human health.
KW - ACE-I activity
KW - Antioxidant activity
KW - Bioactive peptides
KW - Chickpea protein hydrolysates
KW - Functional properties
KW - Value added
UR - http://www.scopus.com/inward/record.url?scp=84861996597&partnerID=8YFLogxK
U2 - 10.1002/jsfa.5570
DO - 10.1002/jsfa.5570
M3 - Artículo
SN - 0022-5142
VL - 92
SP - 1974
EP - 1981
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
IS - 9
ER -