TY - CHAP
T1 - Angiogenesis analysis by in vitro coculture assays in transwell chambers in ovarian cancer
AU - Flores-Pérez, Ali
AU - Rincón, Dolores Gallardo
AU - Ruiz-García, Erika
AU - Echavarria, Raquel
AU - Marchat, Laurence A.
AU - Álvarez-Sánchez, Elizbeth
AU - López-Camarillo, César
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media LLC.
PY - 2018
Y1 - 2018
N2 - Angiogenesis is an important biological process in tumor growth and metastasis of tumor cells, and it has been associated with poor clinical outcomes in ovarian cancer. In vitro assays are useful tools for understanding the complex mechanisms of angiogenesis under a variety of conditions. Capillary-like formation and transwell migration assays are two of the most common techniques used in angiogenesis research. Here, we show an easy coculture model to study the role of microRNAs on angiogenesis that combines tube formation and cell migration assays. Recently, we reported that miR-204 is repressed in breast cancer and restoration in cancer cell lines results in angiogenesis inhibition. Here, we restored the expression of miR-204 by transfection of precursor molecule in the tumorigenic SKOV3 ovarian cancer cell line, and analyzed the effects in cell migration, invasion, and tube formation of endothelial cells using matrigel-coated transwell chambers.
AB - Angiogenesis is an important biological process in tumor growth and metastasis of tumor cells, and it has been associated with poor clinical outcomes in ovarian cancer. In vitro assays are useful tools for understanding the complex mechanisms of angiogenesis under a variety of conditions. Capillary-like formation and transwell migration assays are two of the most common techniques used in angiogenesis research. Here, we show an easy coculture model to study the role of microRNAs on angiogenesis that combines tube formation and cell migration assays. Recently, we reported that miR-204 is repressed in breast cancer and restoration in cancer cell lines results in angiogenesis inhibition. Here, we restored the expression of miR-204 by transfection of precursor molecule in the tumorigenic SKOV3 ovarian cancer cell line, and analyzed the effects in cell migration, invasion, and tube formation of endothelial cells using matrigel-coated transwell chambers.
KW - Angiogenesis
KW - Co-culture assay
KW - HUVEC
KW - SKOV3 ovarian cancer
KW - Transwell chambers
UR - http://www.scopus.com/inward/record.url?scp=85032669588&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7435-1_13
DO - 10.1007/978-1-4939-7435-1_13
M3 - Capítulo
C2 - 29086377
AN - SCOPUS:85032669588
T3 - Methods in Molecular Biology
SP - 179
EP - 186
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
