TY - JOUR
T1 - Analysis of polymorphisms and haplotypes in genes associated with vascular tone, hypertension and oxidative stress in Mexican-Mestizo women with severe preeclampsia
AU - Coral-Vázquez, Ramón Mauricio
AU - Romero Arauz, Juan Fernando
AU - Canizales-Quinteros, Samuel
AU - Coronel, Agustín
AU - Valencia Villalvazo, Elith Yazmin
AU - Hernández Rivera, Jaime
AU - Ramírez Regalado, Belem
AU - Rojano Mejía, David
AU - Canto, Patricia
N1 - Funding Information:
This study was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT), México (grant: SALUD-2007-C01-69693 ). E.Y. Valencia Villalvazo was supported by a fellowship award from CONACYT .
PY - 2013/5
Y1 - 2013/5
N2 - Objective: Several studies have reported the association of genes related to vascular tone, hypertension, oxidative stress and preeclampsia. We investigated the possible association among three polymorphisms in eNOS (as well their haplotypes): one of MTHFR, one of GSTP1 and one of AGT, with severe preeclampsia in Mexican-Mestizo women. Methods: Two hundred thirty women with severe preeclampsia and 350 control subjects were genotyped; for rs2070744 and rs1799983 of eNOS, rs1801133 of MTHFR, rs1695 of GSTP1 and rs699 of AGT we used real-time PCR allelic discrimination and for VNTR of eNOS, PCR. Allele frequency differences were assessed by χ2. Logistic regression was used to test for associations and for haplotype frequencies using Haploview 4.2. Results: Genotypic and allelic distribution of the polymorphisms was similar between cases and controls; likewise, haplotype frequencies of the three polymorphisms of eNOS did not differ significantly. Conclusions: To our knowledge, this is the first time that these polymorphisms have been analyzed together and exclusively in women with severe preeclampsia. However, we did not find an association between polymorphisms of eNOS, MTHFR, GSTP1 and AGT with severe preeclampsia in our population. Additionally, we observed differences in the distribution of the alleles and genotypes of these polymorphisms in our population in comparison to those described in other ethnic groups.
AB - Objective: Several studies have reported the association of genes related to vascular tone, hypertension, oxidative stress and preeclampsia. We investigated the possible association among three polymorphisms in eNOS (as well their haplotypes): one of MTHFR, one of GSTP1 and one of AGT, with severe preeclampsia in Mexican-Mestizo women. Methods: Two hundred thirty women with severe preeclampsia and 350 control subjects were genotyped; for rs2070744 and rs1799983 of eNOS, rs1801133 of MTHFR, rs1695 of GSTP1 and rs699 of AGT we used real-time PCR allelic discrimination and for VNTR of eNOS, PCR. Allele frequency differences were assessed by χ2. Logistic regression was used to test for associations and for haplotype frequencies using Haploview 4.2. Results: Genotypic and allelic distribution of the polymorphisms was similar between cases and controls; likewise, haplotype frequencies of the three polymorphisms of eNOS did not differ significantly. Conclusions: To our knowledge, this is the first time that these polymorphisms have been analyzed together and exclusively in women with severe preeclampsia. However, we did not find an association between polymorphisms of eNOS, MTHFR, GSTP1 and AGT with severe preeclampsia in our population. Additionally, we observed differences in the distribution of the alleles and genotypes of these polymorphisms in our population in comparison to those described in other ethnic groups.
KW - AGT
KW - ENOS
KW - GSTP1
KW - MTHFR
KW - Mexican-Mestizo women
KW - Polymorphisms
KW - Severe preeclampsia
UR - http://www.scopus.com/inward/record.url?scp=84884756563&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2012.12.016
DO - 10.1016/j.clinbiochem.2012.12.016
M3 - Artículo
C2 - 23333443
SN - 0009-9120
VL - 46
SP - 627
EP - 632
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 7-8
ER -