Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico

María F. Jiménez-Becerril, Sanjuana Hernández-Delgado, Myrna Solís-Oba, Juan M. González Prieto

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

© 2016 Informa UK Limited, trading as Taylor & Francis Group. The current understanding of the genetic diversity of the phytopathogenic fungus Ustilago maydis is limited. To determine the genetic diversity and structure of U. maydis, 48 fungal isolates were analyzed using mitochondrial simple sequence repeats (SSRs). Tumours (corn smut or ‘huitlacoche’) were collected from different Mexican states with diverse environmental conditions. Using bioinformatic tools, five microsatellites were identified within intergenic regions of the U. maydis mitochondrial genome. SSRMUM4 was the most polymorphic marker. The most common repeats were hexanucleotides. A total of 12 allelic variants were identified, with a mean of 2.4 alleles per locus. An estimate of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance component is within states (84%), with moderate genetic differentiation between states (16%) (FST= 0.158). A dendrogram generated using the unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure grouped the U. maydis isolates into two subgroups (K = 2) based on their shared SSRs.
Original languageAmerican English
Pages (from-to)1-8
Number of pages0
JournalMitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
DOIs
StatePublished - 2 Jan 2018

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