TY - JOUR
T1 - Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico
AU - Jiménez-Becerril, María F.
AU - Hernández-Delgado, Sanjuana
AU - Solís-Oba, Myrna
AU - González Prieto, Juan M.
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/1/2
Y1 - 2018/1/2
N2 - The current understanding of the genetic diversity of the phytopathogenic fungus Ustilago maydis is limited. To determine the genetic diversity and structure of U. maydis, 48 fungal isolates were analyzed using mitochondrial simple sequence repeats (SSRs). Tumours (corn smut or ‘huitlacoche’) were collected from different Mexican states with diverse environmental conditions. Using bioinformatic tools, five microsatellites were identified within intergenic regions of the U. maydis mitochondrial genome. SSRMUM4 was the most polymorphic marker. The most common repeats were hexanucleotides. A total of 12 allelic variants were identified, with a mean of 2.4 alleles per locus. An estimate of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance component is within states (84%), with moderate genetic differentiation between states (16%) (FST= 0.158). A dendrogram generated using the unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure grouped the U. maydis isolates into two subgroups (K = 2) based on their shared SSRs.
AB - The current understanding of the genetic diversity of the phytopathogenic fungus Ustilago maydis is limited. To determine the genetic diversity and structure of U. maydis, 48 fungal isolates were analyzed using mitochondrial simple sequence repeats (SSRs). Tumours (corn smut or ‘huitlacoche’) were collected from different Mexican states with diverse environmental conditions. Using bioinformatic tools, five microsatellites were identified within intergenic regions of the U. maydis mitochondrial genome. SSRMUM4 was the most polymorphic marker. The most common repeats were hexanucleotides. A total of 12 allelic variants were identified, with a mean of 2.4 alleles per locus. An estimate of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance component is within states (84%), with moderate genetic differentiation between states (16%) (FST= 0.158). A dendrogram generated using the unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure grouped the U. maydis isolates into two subgroups (K = 2) based on their shared SSRs.
KW - Basidiomycete
KW - genetic diversity
KW - mitochondrial SSR
KW - plant pathogen
UR - http://www.scopus.com/inward/record.url?scp=84990924583&partnerID=8YFLogxK
U2 - 10.1080/24701394.2016.1229776
DO - 10.1080/24701394.2016.1229776
M3 - Artículo
C2 - 27728988
SN - 2470-1394
VL - 29
SP - 1
EP - 8
JO - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
JF - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
IS - 1
ER -