TY - JOUR
T1 - Analysis of microbial diversity of inocula used in a five-face parallelepiped and standard microbial fuel cells
AU - Ortega-Martínez, A. C.
AU - Juárez-López, K.
AU - Solorza-Feria, O.
AU - Ponce-Noyola, M. T.
AU - Galindez-Mayer, J.
AU - Rinderknecht-Seijas, N.
AU - Poggi-Varaldo, H. M.
N1 - Funding Information:
The authors want to express their gratitude to Dr. E.A. Veziroglu, Editor-in-Chief and Dr. Arunabha Kundu, Assistant Subject Editor of the International Journal of Hydrogen Energy, as well as the anonymous Reviewers, for their insightful comments, criticisms, and suggestions, that helped to significantly improve the revised manuscript. The authors also wish to thank ICYTDF (Project PICCO 10-28) and CINVESTAV-IPN for financial support to this research. Areli del C. Ortega-Martinez received a graduate scholarship from CONACYT, Mexico. The excellent technical help with molecular biology analysis of Ms Ana Lilia Tirado-Chamú (BSBiochemEng) from IBT-UNAM, Mr. Rafael Hernández-Vera and personnel of the Environmental of Biotechnology and Renewable Energy R&D Group CINVESTAV-IPN is greatly appreciated.
PY - 2013
Y1 - 2013
N2 - This work had a double purpose: (i) to study the effect of sulphate-reducing (SR-In) and enriched (E-In) inocula on the characteristics of one-chamber standard microbial fuel cell (MFC-S) and parallelepiped cell and (ii) to analyze the bacterial communities in cells operated with either SR-In or E-In. The MFC-S of 150 mL consisted of one-chamber plexiglass cell with electrodes separated 7.8 cm. The MFC-P consisted of a parallelepiped built in plexiglass with a liquid volume of 270 mL. Five faces of this cell were fitted with 'sandwich' cathode-membrane-anode assemblages (CMA). The values of internal resistance (Rint) were 4602 and 687 Ω, for the MFC-S loaded with SR-In and E-In, respectively. The values of Rint were 400 and 84, and 292 and 80 Ω for the faces connected in series and parallel and the MFC-P loaded with SR-In and E-In, respectively. Parallel connection of cell faces also significantly improved the electrochemical characteristics of the P cell (higher powers). In general, use of E-In in both types of MFC lead to improved power densities compared to SR-In. Molecular biology analysis of microbial communities showed that the E-In was less diverse than SR-In (in terms of phyla). An electrochemically active bacterium Geovibrio ferrireducens belonging to phylum Deferribacteres was found in the E-In. Predominance of Deferribacteres was observed in the E-In. Members of this phylum were not found in the SR-In.
AB - This work had a double purpose: (i) to study the effect of sulphate-reducing (SR-In) and enriched (E-In) inocula on the characteristics of one-chamber standard microbial fuel cell (MFC-S) and parallelepiped cell and (ii) to analyze the bacterial communities in cells operated with either SR-In or E-In. The MFC-S of 150 mL consisted of one-chamber plexiglass cell with electrodes separated 7.8 cm. The MFC-P consisted of a parallelepiped built in plexiglass with a liquid volume of 270 mL. Five faces of this cell were fitted with 'sandwich' cathode-membrane-anode assemblages (CMA). The values of internal resistance (Rint) were 4602 and 687 Ω, for the MFC-S loaded with SR-In and E-In, respectively. The values of Rint were 400 and 84, and 292 and 80 Ω for the faces connected in series and parallel and the MFC-P loaded with SR-In and E-In, respectively. Parallel connection of cell faces also significantly improved the electrochemical characteristics of the P cell (higher powers). In general, use of E-In in both types of MFC lead to improved power densities compared to SR-In. Molecular biology analysis of microbial communities showed that the E-In was less diverse than SR-In (in terms of phyla). An electrochemically active bacterium Geovibrio ferrireducens belonging to phylum Deferribacteres was found in the E-In. Predominance of Deferribacteres was observed in the E-In. Members of this phylum were not found in the SR-In.
KW - Enriched inoculum
KW - Internal resistance
KW - Microbial fuel cell
UR - http://www.scopus.com/inward/record.url?scp=84883778950&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2013.02.023
DO - 10.1016/j.ijhydene.2013.02.023
M3 - Artículo
AN - SCOPUS:84883778950
SN - 0360-3199
VL - 38
SP - 12589
EP - 12599
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 28
ER -
