TY - JOUR
T1 - Analysis of arsenical metabolites in biological samples.
AU - Hernandez-Zavala, Araceli
AU - Drobna, Zuzana
AU - Styblo, Miroslav
AU - Thomas, David J.
PY - 2009/11
Y1 - 2009/11
N2 - Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.
AB - Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.
UR - http://www.scopus.com/inward/record.url?scp=84879792280&partnerID=8YFLogxK
M3 - Artículo
SN - 1934-9262
VL - 42
SP - 4.33.1-4.33.17
JO - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
JF - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
ER -