TY - JOUR
T1 - An anti-inflammatory oligopeptide produced by Entamoeba histolytica down-regulates the expression of pro-inflammatory chemokines
AU - Utrera-Barillas, Dolores
AU - Velazquez, Juan R.
AU - Enciso, Antonio
AU - Cruz, Samira Muñoz
AU - Rico, Guadalupe
AU - Curiel-Quesada, Everardo
AU - Teran, Luis M.
AU - Kretschmer, Roberto R.
PY - 2003/10
Y1 - 2003/10
N2 - Axenically grown Entamoeba histolytica produces a pentapeptide (Met-Gln-Cys-Asn-Ser) with anti-inflammatory properties that, among others, inhibits the in vitro and in vivo locomotion of human monocytes, sparing polymorphonuclear leucocytes from this effect [hence the name originally given: Monocyte Locomotion Inhibitory Factor (MLIF)]. A synthetic construct of this peptide displays the same effects as the native material. We now added MLIF to resting and PMA-stimulated cells of a human monocyte cell line and measured the effect upon mRNA and protein expression of pro-inflammatory chemokines (RANTES, IP-10, MIP-1α, MIP-1β, MCP-1, IL-8, I-309 and lymphotactin) and the shared CC receptor repertoire. The constitutive expression of these chemokines and the CC receptors was unaffected, whereas induced expression of MIP-1α, MIP-1β, and I-309, and that of the CCR1 receptor - all involved in monocyte chemotaxis - was significantly inhibited by MLIF. This suggests that the inhibition of monocyte functions by MLIF may not only be exerted directly on these cells, but also - and perhaps foremost - through a conglomerate down-regulation of endogenous pro-inflammatory chemokines.
AB - Axenically grown Entamoeba histolytica produces a pentapeptide (Met-Gln-Cys-Asn-Ser) with anti-inflammatory properties that, among others, inhibits the in vitro and in vivo locomotion of human monocytes, sparing polymorphonuclear leucocytes from this effect [hence the name originally given: Monocyte Locomotion Inhibitory Factor (MLIF)]. A synthetic construct of this peptide displays the same effects as the native material. We now added MLIF to resting and PMA-stimulated cells of a human monocyte cell line and measured the effect upon mRNA and protein expression of pro-inflammatory chemokines (RANTES, IP-10, MIP-1α, MIP-1β, MCP-1, IL-8, I-309 and lymphotactin) and the shared CC receptor repertoire. The constitutive expression of these chemokines and the CC receptors was unaffected, whereas induced expression of MIP-1α, MIP-1β, and I-309, and that of the CCR1 receptor - all involved in monocyte chemotaxis - was significantly inhibited by MLIF. This suggests that the inhibition of monocyte functions by MLIF may not only be exerted directly on these cells, but also - and perhaps foremost - through a conglomerate down-regulation of endogenous pro-inflammatory chemokines.
KW - Chemokines
KW - E. histolytica peptide
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=1542616268&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3024.2003.00657.x
DO - 10.1111/j.1365-3024.2003.00657.x
M3 - Artículo
SN - 0141-9838
VL - 25
SP - 475
EP - 482
JO - Parasite Immunology
JF - Parasite Immunology
IS - 10
ER -